绒山羊次级毛囊生长调控基因的筛选

doi:10.11843/j.issn.0366-6964.2015.10.005

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (10): 1733-1740.doi: 10.11843/j.issn.0366-6964.2015.10.005

绒山羊次级毛囊生长调控基因的筛选

李晓燕^#，张璐^#，王乐乐，邬娇，宋慧子，涂藩镜，张燕军^*

（内蒙古农业大学动物科学学院，动物遗传育种与繁殖自治区重点实验室，呼和浩特 010018）

收稿日期:2015-03-17 出版日期:2015-10-23 发布日期:2015-10-19
通讯作者: 张燕军，博士，副教授，主要从事绒山羊遗传育种研究，E-mail：imauzyj@163.com
作者简介:李晓燕（1990-），女，内蒙古锡林郭勒盟人，硕士生，主要从事绒山羊遗传育种研究，E-mail:lxy12898159@126.com；张璐（1989-），女，内蒙古四子王旗人，硕士生，主要从事绒山羊遗传育种研究，E-mail:zl63412732@163.com。李晓燕和张璐为并列第一作者
基金资助:
国家自然科学基金项目（31260539；31201773）；内蒙古农业大学优秀青年科学基金（2014XYQ-1 ）；内蒙古农业大学学生科技创新基金项目（2014-1 ）；国家现代绒毛用羊产业技术体系建设项目（CARS-40-05）

Selection of Relative Gene during Secondary Hair Follicle Growth in Cashmere Goat

LI Xiao-yan^#，ZHANG Lu^#，WANG Le-le，WU Jiao，SONG Hui-zi，TU Fan-jing，ZHANG Yan-jun^*

（Inner Mongolia Key Laboratory of Animal Genetics，Breeding and Reproduction，College of Animal Science，Inner Mongolia Agricultural University，Hohhot 010018，China）

Received:2015-03-17 Online:2015-10-23 Published:2015-10-19

摘要/Abstract

摘要：

旨在筛选绒山羊次级毛囊生长调控基因。本研究以遗传背景相似，有次级毛囊的绒山羊和无次级毛囊的奶山羊为试验动物，利用Illumina Hiseq2000 对4只绒山羊与4只奶山羊3、6、9、12月的32个皮肤样品进行RNA-Seq测序。测序共获得38G数据，共筛选出20 780个基因。差异表达基因分析表明，绒山羊与奶山羊之间在不同时期共有2 409个差异表达基因，其中上调差异表达基因1 175个，下调差异表达基因1 234个，绒山羊之间在不同生长时期差异表达基因共550个，其中上调差异表达基因302个，下调差异表达基因248个。GO功能注释分类显示，差异表达基因主要在细胞与细胞部分、细胞器部分、结合、催化、生物调控、细胞过程、代谢过程等功能富集。 KEGG pathway分析发现，42个差异表达基因富集在与毛囊生长相关的WNT、SHH、TGFβ、TNF、MAPK和Notch信号通路中，由此推测其可能为次级毛囊生长相关基因。本研究为深入开展次级毛囊生长相关基因功能验证提供丰富的资源，为揭示次级毛囊生长调控机制提供重要基础，为绒毛产量及品质的提升提供理论依据。

关键词: 绒山羊, 次级毛囊, 调控基因, 筛选

Abstract:

In order to select the genes which regulate the growth of secondary hair follicle in cashmere goat.This study choose cashmere goat and dairy goats to be the material.Because these 2 goats get the similar genetic background，but significant differences traits of the secondary follicle.An Illumina Hiseq2000 platform was used to sequence the 32 skin samples from 4 cashmere goat and 4 dairy goat on March，June，September and December.The Sequencing obtained 38G data，check out 20 780 goats genes.We identified 2 409 differentially expressed genes between cashmere goat and dairy goat in the same period，1 175 cashmere goat genes were up regulated compared with dairy goat，1 234 were down regulated.And there were a total of 550 differentially expressed genes between different growth periods in cashmere goat，302 cashmere goat genes were up regulated，248 were down regulated.GO functional annotations showed that the main differentially expressed genes appear in the cells and cell parts，organs parts，binding，biological regulation，cellular process.KEGG pathway analysis found that 42 differentially expressed genes appear in WNT，SHH，TGF β，TNF，MAPK and Notch signaling pathways which intimately associated with hair follicle growth.This study provided valuable information to verify secondary follicle growth related gene function，provided an important basis to reveal the secondary follicle growth mechanism and provided the theory to increase production and quality of cashmere.

Key words: cashmere gaot, secondary follicle, regulatory gene, select

中图分类号:

S827.2

李晓燕，张璐，王乐乐，邬娇，宋慧子，涂藩镜，张燕军. 绒山羊次级毛囊生长调控基因的筛选[J]. 畜牧兽医学报, 2015, 46(10): 1733-1740.

LI Xiao-yan，ZHANG Lu，WANG Le-le，WU Jiao，SONG Hui-zi，TU Fan-jing，ZHANG Yan-jun. Selection of Relative Gene during Secondary Hair Follicle Growth in Cashmere Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1733-1740.

参考文献

［1］ALONSO L，FUCHS E.The hair cycle［J］.J Cell Sci, 2006,119:391-393.
［2］YANG J B，GAN S Q，YANG Y L.ILK gene cloning and expression of hair follicle growth period of sheep［J］.Genetic,2012,34(6):179-125.
［3］ZHU B，XU T,YUAN J L，et al.Transcriptome sequencing reveals differences between primary and secondary hair follicle-derived dermal papilla cells of the Cashmere goat (Capra hircus)［J］.PLoS ONE,2013,8(9)：e76282.
［4］李金泉，尹俊，周欢敏.绒山羊绒毛生长调控机理研究进展［J］.中国草食动物,2001,3(3):41-44.
LI J Q，YIN J，ZHOU H M.The research progress of cashmere goat growth regulation mechanism ［J］.Herbivores of China,2001,3(3):41-44.(in Chinese)
［5］张春兰.内蒙古阿尔巴斯白绒山羊毛囊生长周期性变化与KAP6 cDNA的特征分析［D］.呼和浩特:内蒙古农业大学，2003
ZHANG C L.Inner Mongolia Albath White Cashmere goat hair follicle growth periodic and KAP6 cDNA characteristics analysis［D］.Hohhot：Inner Mongolia Agricultural University，2003.(in Chinese)
［6］ENSHELL SEIJFFERS D，LINDON C，KASHIWAG M，et al.beta-catenin activity in the dermal papilla regulates morphogenesis and regeneration of hair［J］. Dev Cell,2010,18 (4):633-642.
［7］WOO W M，ZHEN H，ORO A.Shh maintains dermal papilla identity and hair morphogenesis via a Noggin-Shh regulatory loop［J］.Genes Dev,2012(26):1235-1246.
［8］TEICHERT A E，ELALIEH H，ELIAS P M，et al.Overexpression of Hedgehog signaling is associated with epidermal tumor formation invitamin d receptor-null mice［J］.Sci Int Dermatol,2011:2289-2297.
［9］MOU C Y，JACKSON B，SCHNEIDER P,et al.Generation of the primary hair follicle pattern ［J］.PNAS,2006(24):9075-9080.
［10］UEKI M K，YUKO O，JUNKO O，et al.Hair cycle resting phase is regulated by cyclic epithelial FGF18 signaling［J］. J Int Dermatol,2012，132(5):1338-1345.
［11］HUH S H,NÄRHI K,LINDFORS P H,et al，Fgf20 governs formation of primary and secondary dermal condensations in developing hair follicles［J］.Genes Dev,2013，27(4):450-458.
［12］KARLSSON L，BONDJERS C，BETSHOLTZ C.Roles for PDGF-A and sonic hedgehog in development of mesenchymal components of the hair follicle［J］.Development,1999,126:2611-2621
［13］ULLRICH R，PAUS R.Molecular principles of hair follicle induction and morphogenesis［J］.Biol Essays，2005，27(3)：247-261.
［14］SOMA T，OGO M.Analysis of apoptotic cell death in human hair follicles in vivo and in vitro［J ］.J Int Dermatol，1998,111：948-954.
［15］OSHIMORI1 N，FUCHS E.Paracrine TGF-β signaling counterbalances BMP-mediated repression in hair follicle stem cell activation［J］，Cell Stem Cell,2012(10):63-75.
［16］MYLLYLA R M，HAAPASAARI K M，PALATSI R，et al.Multiple miliary osteoma cutis is adistinct disease entity：four case reports and review of the literature［J］.Br J Dermatol，2011,164:544-552.
［17］MIKKOLA M L.TNF superfamily in skin appendage development［J］.Cytokine Growth Factor Rev，2008（19）：219-230.
［18］LEI M X，BAI X F，YANG T，et al.Gsdma3 is a new factor needed for TNF-α mediated apoptosis signal pathway in mouse skin keratinocytes［J］. Histochem Cell Biol，2012，138:385-396.
［19］CROWE R，HENRIQUE D，HOROWICZ D，et al.A new role for Notch and Delta in cell fate decisions patterning the feather array ［J］.Development，1998，125(4):767-775.
［20］ZHANG Y，YU J，SHI C Y,et al.Lef1 contributes to the differentiation of bulge stem cells by nuclear translocation and cross-talk with the notch signaling pathway［J］.Int J Med Sci,2013 10(6):738-746.
［21］许腾.绒山羊皮肤绒毛生长期与静息期基因差异表达的研究［D］.呼和浩特：内蒙古大学，2013.
XU T.Research of differentially expressed genes of cashmere goat hair follicles anagen and telogen［D］.Hohhot：Inner Mongolia University,2013.(in Chinese)
［22］王娟，杨雪艳，王红艳，等.Vangl2及其相关基因与神经管畸形的关系［J］.生命的化学，2010，30（5）：730-733.
WANG J，YANG X Y，WANG H Y，et al.Progress of Vangl2 and related genes in NTD［J］.Chemistry of Life，2010，30（5）：730-733.(in Chinese)
［23］罗丽敏，王俐.Wnt通路抑制因子Sfrp的研究进展［J］.医学综述，2007，13（10）：729-731.
LUO L M,WANG L.The research progress of Wnt pathways inhibitory factor Sfrp［J］.Medical Recapitulate,2007,13(10):729-731.（in Chinese）
［24］胡毓安,赵春杰.Wif1在神经系统发育中的研究进展［J］.浙江大学学报（医学版），2010,39（1）：93-96.
HU Y A，ZHAO C J.The research progress of Wif1 in nervous system development［J］.Journal of Zhejiang University(Medical Sciences),2010,39(1)：93-96.(in Chinese)

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绒山羊次级毛囊生长调控基因的筛选

Selection of Relative Gene during Secondary Hair Follicle Growth in Cashmere Goat

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