老龄牦牛睾丸细胞外基质相关蛋白的分布特征

doi:10.11843/j.issn.0366-6964.2015.09.023

摘要/Abstract

摘要：

探索细胞外基质相关蛋白在老龄牦牛睾丸的分布特征。应用组织化学方法比较、观察9头健康老龄牦牛和10头青年牦牛睾丸组织结构特点及层黏连蛋白（LN）、Ⅳ型胶原（Col Ⅳ）和硫酸乙酰肝素糖蛋白（HSPG）的分布特征。结果显示：光镜下，老龄牦牛生精上皮部分或完全退化，生精小管固有膜及间质胶原纤维及网状纤维较青年牦牛丰富；老龄睾丸间质血管及生精小管固有膜中AB-PAS阳性反应较青年牦牛增强。数据统计表明，老龄牦牛Sertoli细胞及Leydig细胞数均明显减少，生精小管横截面积以及平均间质组织面积极显著大于青年牦牛(P<0.01)。免疫组织化学显示，LN在老龄牦牛睾丸Sertoli细胞和肌样细胞表达与青年牦牛相近，LN在生精细胞表达降低，而在Leydig细胞几乎无表达；Col Ⅳ在不同年龄牦牛睾丸组织表达位置及强弱相似，但其平均吸光度检测结果无统计学差异；老龄牦牛睾丸组织HSPG主要在Leydig细胞表达降低，平均吸光度检测统计极显著低于青年牦牛（P<0.01）；同一年龄段LN 、Col Ⅳ和HSPG表达无明显差异。高原环境中，老龄牦牛生精上皮退化伴随着间质成分增加、间质面积增大、Leydig细胞及Sertoli细胞数量减少等形态学变化；睾丸组织Col Ⅳ分泌增加且胶原纤维合成增强，LN和HSPG的显著降低可能影响Leydig细胞合成分泌能力。

关键词: 老龄牦牛, 睾丸, 层黏连蛋白, 胶原蛋白, 硫酸乙酰肝素糖蛋白, 免疫组织化学

Abstract:

This study was conducted to characterize the structure and distribution of extracellular matrix(ECM) proteins of testis in aging yak testis.The testis from 9 aging and 10 young adult yak were prepared for distribution investigation of laminin(LN)，type IV collagen(ColⅣ) and heparan sulfate proteoglycans(HSPG) by immunohistochemistry and histochemistry methods.The observations made with the light microscope showed obvious morphological changes of aging，there were partly progressive regressed in the seminiferous epithelium and the volume of interstitial tissues has further increased and abundant with collagen and reticular fiber，and also there were AB-PAS positive stain in aging yak seminiferous basement and blood capillary.Statistical data showed that the number of Sertoli cells and Leydig cells in aging yak testis were reduced obviously，but the cross-sectional area of the seminiferous tubules and average interstitial tissue areas were more larger than which in the young yak testis.Immunostaining analysis appeared that the LN was present similarity in Sertoli cells and peritubular myoid cells in the aging and young adult yak.The relative expression of LN in spermatogenic cells was decreased and almost no detected in aging yak Leydig cells(P<0.01).By contrast，the distribution of Col Ⅳ without statistical differences in yak testis at different ages.The relative expression of HSPG in the aging yak testis tissue was more lower than which in young adult yak，and the strong immunoreactivity for HSPG was seen in the Leydig cells(P<0.01).In addition，the average absorbance of LN，Col Ⅳand HSPG expression was no obvious difference between different ages.All the elements of the intralobular interstitial space may undergo degenerative changes in aging yak which live in plateau environment，the secretion of Col Ⅳ increased company with the synthesis of collagen fiber reinforced，and the distribution of ECM proteins LN and HSPG were significantly decreased may closely related with the alterations of Leydig cells′ synthesis and secretion ability.

Key words: aging yak, testis, laminin, collagenⅣ, heparan sulfate proteoglycans, immunohistochemistry

中图分类号:

S852.162

袁莉刚，朱峻峰，谷来凤，陈国娟. 老龄牦牛睾丸细胞外基质相关蛋白的分布特征[J]. 畜牧兽医学报, 2015, 46(9): 1656-1662.

YUAN Li-gang，ZHU Jun-feng，GU Lai-feng，CHEN Guo-juan. Distribution of Extracellular Matrix Components in Aging Yak Testis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1656-1662.

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