维生素E对小鼠体内精子品质及精子DNA的保护作用

doi:10.11843/j.issn.0366-6964.2015.02.022

摘要/Abstract

摘要：

旨在研究维生素E对小鼠精子DNA的保护作用及对精液中抗氧化物酶活性的影响。以小鼠为动物模型，选日龄、大小相同的健康雄性小白鼠50只，随机分成5组，在试验组小鼠的每千克日粮中分别添加4、8、12、16 g 的维生素E，对照组添加量为0，饲喂30 d后，通过颈椎脱臼处死小鼠，解剖后通过附睾和曲精细管获得小鼠精液。通过单细胞凝胶电泳及相关检测试剂盒检测分析各组间精液DNA的完整性、精子活力、密度、畸形率、精浆中的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH）、谷胱甘肽还原酶（GR）和过氧化氢酶（CAT）的活性。结果表明：每千克日粮中维生素E的添加量为12 g 时，0级精子百分率与对照组相比差异显著(P<0.05)；添加量为8~16 g 时，精子活力与对照组相比差异显著(P<0.05)；GR和SOD酶的活性与对照组相比差异显著(P<0.05)。每千克日粮中维生素E添加量为12~16 g 时，CAT酶的活性与对照组相比差异显著(P<0.05)。综上表明，在饲粮中添加维生素E浓度为12 g•kg^-1时可以明显提高小鼠精液中CAT、GR、GSH和SOD酶的活性及精子DNA的完整性。

关键词: 小鼠, 维生素E, 精子, DNA完整性

Abstract:

This study aimed to investigate the effects of vitamin E added in diet on sperm DNA integrity and antioxidant activities of semen in mice.50 mice divided into 5 groups were fed in the experiment，in which vitamin E was added in diet with the different content of 0（Control），4，8，12 and 16 g•kg^-1，respectively.The mice were killed by cervical vertebra dislocation and dissected after 30 days when semen was obtained from epididymis and seminiferous tubules.The sperm motility，density，deformity rate and DNA integrity were detected by single cell gel electrophoresis(SCGE) assay and test kits，as well as the antioxidant activities including superoxide dismutase (SOD)，glutathione peroxidase (GSH)，glutathione reductase (GR) and catalase (CAT).The percentage of “grade 0” sperm in diet supplemented with 12 g•kg^-1 vitamin E was significantly higher than that of other treatment groups (P<0.05).The sperm motility was significantly improved (P<0.05) and the levels of GR and SOD in seminal plasma were significantly higher (P<0.05) by the supplementing with 8-16 g•kg^-1 vitamin E in diet than the control，respectively.The CAT activity in seminal plasma reached the highest level with supplementation of 12-16 g•kg^-1 vitamin E in diet，when compared to the others (P<0.05).In conclusion，we could infer that 12 g•kg^-1 vitamin E added could obviously improve the sperm DNA integrity and the antioxidant activities of CAT，GR，GSH and SOD in seminal plasma.

Key words: mice, vitamin E, sperm, DNA integrity

中图分类号:

Q492.6

赵宪林，曹少杰，赵春平，郝瑞杰，桂林生，昝林森. 维生素E对小鼠体内精子品质及精子DNA的保护作用[J]. 畜牧兽医学报, 2015, 46(2): 332-338.

ZHAO Xian-lin，CAO Shao-jie,ZHAO Chun-ping，HAO Rui-jie,GUI Lin-sheng，ZAN Lin-sen. The Protection of Vitamin E on Quality and Integrity of Mice Semen in vivo[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 332-338.

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