畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (2): 317-322.doi: 10.11843/j.issn.0366-6964.2015.02.020

• 临床兽医 • 上一篇    下一篇

小型猪复合麻醉颉颃剂对大鼠各脑区p-p38蛋白及c-myc mRNA表达的影响

李妍#,侯金龙#,牛栋梁, 姜胜,时静,范宏刚,王洪斌*   

  1. (东北农业大学动物医学学院,哈尔滨 150030)
  • 收稿日期:2014-04-10 出版日期:2015-02-23 发布日期:2015-02-25
  • 通讯作者: 王洪斌,教授,博士生导师,E-mail:neau1940@yahoo.com.cn
  • 作者简介:李妍(1988-),女,河南安阳人,硕士,主要从事麻醉与镇痛研究,E-mail:liyan8835@yeah.net;侯金龙(1986-),男,助理实验师,主要从事动物麻醉研究,E-mail:2680310282@qq.com。二人对本文同等贡献,共为第一作者
  • 基金资助:

    国家自然科学基金项目(31272617;31001092)

Effect of the Antagonist for the Combined Anaesthetic for Miniature Pigs on p-p38 Protein and c-myc Gene in Brain Regions of Rats

LI Yan#,HOU Jin-long#,NIU Dong-liang,JIANG Sheng,SHI Jing,FAN Hong-gang,WANG Hong-bin*   

  1. (College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China)
  • Received:2014-04-10 Online:2015-02-23 Published:2015-02-25

摘要:

旨在研究小型猪复合麻醉颉颃剂对大鼠不同脑区p-p38蛋白及c-myc mRNA表达的影响,探讨小型猪复合麻醉颉颃剂的催醒机制。将18只大鼠随机分为C组(对照组)、J组(麻醉颉颃剂组)。J组又分为2个亚组,即J1组(注射小型猪专用复合麻醉颉颃剂5 min)、J2 组(注射小型猪专用复合麻醉颉颃剂1 h)。各组大鼠到达相应的时间点后断头处死,分离大脑皮层、小脑、海马、脑干和丘脑,用Western blot的方法检测p-p38蛋白的表达量,实时荧光定量PCR方法检测c-myc基因mRNA的转录量。试验结果显示大鼠大脑皮层、丘脑和脑干的p-p38蛋白和c-myc mRNA的相对表达量显著升高,而小脑和海马的p-p38蛋白和c-myc mRNA的相对表达量显著降低。综合试验结果,小型猪复合麻醉颉颃剂能够影响p-p38蛋白和c-myc mRNA的表达,这可能是其产生催醒作用的机制之一。

关键词: 小型猪专用复合麻醉颉颃剂, 催醒机制, p-p38蛋白, c-myc mRNA

Abstract:

In order to investigate the effects of antagonist for the Combined Anaesthetic for Miniature Pigs (XFM) on p-p38 protein and c-myc gene in different encephalic region of rats,and discuss the mechanism of XFM antagonist.Eighteen Wistar rats were divided randomly into C (Control group) and J (Antagonist group) group.J group consisted of two subgroups,J1(5 min after rats received the XFM antagonist intraperitoneally) and J2 (1 h after rats received the XFM antagonist intraperitoneally).The brain tissues were collected at each time point.The expression of p-p38 in different brains regions were detected by Western blot.The transcription of c-myc mRNA in different brains regions were detected by real time PCR.The results showed that the expression of p-p38 and c-myc mRNA in cerebral cortex,thalamus and brain stem increased significantly;the expression of p-p38 and c-myc mRNA in cerebellum and hippocampus decreased significantly.These results indicated that p-p38 protein and c-myc gene were affected by the XFM antagonist,this may be one of the antagonism mechanisms.

Key words: antagonist for XFM, antagonism mechanism, p-p38 protein, c-myc mRNA

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