太湖鹅与皖西白鹅胸肌IGF-I 和MSTN的表达及其与屠宰性状相关性分析

doi:10.11843/j.issn.0366-6964.2015.02.005

摘要/Abstract

摘要：

本研究旨在探讨IGF-I、MSTN对鹅骨骼肌生长的影响。以太湖鹅和皖西白鹅为研究素材，采用多重荧光竞争性PCR和酶联免疫方法，测定鹅70日龄胸肌中IGF-I、MSTN的mRNA和蛋白表达情况，并与屠宰性能做相关性分析。结果证实，70日龄时太湖鹅体重、胸肌重极显著小于皖西白鹅，但是胸肌率没有差异，并且品种间在体重和胸肌重上存在性别差异；胸肌IGF-I和MSTN的mRNA、蛋白表达均无品种差异，并且在品种间也不存在性别差异；同一品种的公母鹅相比较，太湖鹅公鹅胸肌IGF-I mRNA表达显著高于母鹅；除了胸肌IGF-I的含量与MSTN的mRNA表达和蛋白含量呈显著正相关，胸肌IGF-I、MSTN的mRNA表达和蛋白含量相互之间均无显著相关性，鹅胸肌IGF-I mRNA表达量和胸肌率、胸肌重、体重呈显著负相关,皖西白鹅胸肌中MSTN的表达与体重呈强的负相关。结果提示，70日龄时鹅胸肌生长正负调节基因IGF-I与MSTN的表达无品种特异性，二者表达水平处于一个动态平衡之中，特别参与了70日龄皖西白鹅的生长调控。

关键词: 鹅, 肌肉, IGF-I , MSTN, 屠宰

Abstract:

The aim of this study was to investigate the effects of IGF-I and MSTN on goose skeletal muscle growth.In this study，the expression of IGF-I and MSTN in breast muscle of Taihu and Wanxi White goose at the age of 70 days were detected with Multiplex Competitive Fluorescent-PCR and Enzyme-linked immunoassay (ELISA) methods，and the correlations between gene expression levels and carcass traits were investigated.The result showed that the body weight and breast muscle weight of Taihu goose were significantly lower than those of Wanxi White goose at the age of 70 days，there was no significant gender difference in breast muscle percentage between the two breeds and there was significant gender difference in body weight and breast muscle weight between the two breeds.There was no breed and gender difference at the mRNA and protein levels of IGF-I and MSTN，and but in Taihu goose,IGF-I mRNA expression in male breast muscle was significantly higher than that of females.There was no significant correlation between mRNA expression and protein content of the two genes.There was a positive correlation between IGF-I and MSTN at the protein level of breast muscle.There was a negative correlation between IGF-I mRNA expression and body weight，breast muscle weight and breast muscle percentage in goose，and a negative correlation between the expression of MSTN and body weight had been detected in Wanxi White goose.The results suggested that the expression levels of genes involving in positive and negative regulation to muscle growth may be in a dynamic balance，special participated in the growth regulation on Wanxi White goose at the age of 70 days.

Key words: goose, muscle, IGF-I, MSTN, slaughter trait

中图分类号:

S835.2

汤青萍，宋迟，胡艳，章双杰，赵东伟，邹剑敏. 太湖鹅与皖西白鹅胸肌IGF-I 和MSTN的表达及其与屠宰性状相关性分析[J]. 畜牧兽医学报, 2015, 46(2): 204-210.

TANG Qing-ping，SONG Chi，HU Yan，ZHANG Shuang-jie，ZHAO Dong-wei，ZOU Jian-min. Expression of IGF-I and MSTN in Taihu and Wanxi White Goose Breast Muscle and Their Correlation with Carcass Traits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 204-210.

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