马立克病病毒感染鸡心组织肿瘤病变与热休克蛋白表达的相关性分析

doi:10.11843/j.issn.0366-6964.2014.12.015

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (12): 2013-2018.doi: 10.11843/j.issn.0366-6964.2014.12.015

马立克病病毒感染鸡心组织肿瘤病变与热休克蛋白表达的相关性分析

裴兰英¹，冯秀娟²，李娟³，李玉保^1*，王守荣¹ ，朱育伟¹，吴伟胜¹

（1.聊城大学农学院，聊城 252059；2.公安部南京警犬研究所，南京 210012；3.扬州大学兽医学院，扬州 225009）

收稿日期:2014-04-23 出版日期:2014-12-23 发布日期:2014-12-23
通讯作者: 李玉保（1978-），男，副教授，博士，主要从事动物分子病理学研究，E-mail：liyubao@lcu.edu.cn
作者简介:裴兰英（1978-），女，讲师，硕士，主要从事动物病理学研究，E-mail：peilanying@lcu.edu.cn
基金资助:
国家自然科学基金（31101787）；山东省自然科学基金（ZR2010CM035）

Relationship between Expression Level of HSPs and Heart Tissue Pathological Lesion of Chickens in Progress of Marek’s Disease

PEI Lan-ying¹，FENG Xiu-juan²，LI Juan³，LI Yu-bao^1*，WANG Shou-rong¹，ZHU Yu-wei¹，WU Wei-sheng¹

(1.College of Agriculture，Liaocheng University，Liaocheng 252059，China；2.Nanjing Police Dog Research Institution of the Ministry of Public Security，Nanjing 210012，China；3.College of Veterinary Medicine，Yangzhou University，Yangzhou 225009，China)

Received:2014-04-23 Online:2014-12-23 Published:2014-12-23

摘要/Abstract

摘要：

通过人工感染建立鸡MD肿瘤模型，研究鸡心病理组织学损伤、热休克蛋白（HSP）组织细胞定位以及HSPs表达水平的动态变化，探讨其相关性。1日龄，攻毒组肌肉注射0.2 mL MDV液（约1 200 PFU），疫苗免疫对照组进行常规MD疫苗注射免疫，于7、14、21、28、35、42及86日龄剖杀试验鸡，利用组织学、免疫组织化学和ELISA等方法，检测心病理组织学损伤、HSPs组织细胞定位以及HSPs表达水平的动态变化。结果如下：在MDV攻毒 14 d后，心组织可见大小不等的淋巴样瘤细胞浸润；HSP90主要分布在心肌纤维之间的肿瘤细胞胞质内，HSP70主要分布于心肌纤维胞质中；攻毒组HSP90含量始终高于空白对照组和疫苗对照组，28日龄后差异极显著（P<0.01)，86日龄时约为两对照组的4.021和3.746倍；攻毒组HSP70含量呈现阶段性升高，35日龄时差异极显著（P<0.01)，整个试验周期内，空白对照组和疫苗对照组HSPs表达量差异不显著。HSP90的组织细胞内定位与肿瘤细胞显著相关，主要位于肿瘤细胞胞质内，整个试验周期HSP90表达量显著高于对照组，有望成为鸡MDV引起的肿瘤疾病发生、发展的判定指标之一。

关键词: 鸡马立克病, 热休克蛋白, 组织学, 免疫组织化学

Abstract:

The tumor model of Marek’s disease (MD) was established by means of artificial MDV infection.Relationship between pathological lesion of heart and distribution，expression level of heat shock proteins (HSPs) were studied during the progress of tumor.At the day 7，14，21，28，35，42 and 86 post artificial infection (PI)，chickens were killed and the hearts were sampled.Pathological lesion of heart and distribution，expression level of HSPs were studied during the progress of tumor by the methods of histopathology，immunohistochemistry and ELISA.Results were as follows：At 14 days of age，obvious pathological damage appeared in the hearts of chickens attacked by MDV，and unequal-sized lymphoid cells infiltration was found among myocardial fiber.HSP90 was mainly located in the cytoplasm of tumor cell and HSP70 was mainly located in the cytoplasm of myocardial fiber.Within the course of MD，the content of HSP90 of infected group was always higher than that of blank control group and vaccine control group，which was significantly higher after 28 day’s，and were 4.021 and 3.746 times higher than the control group at the age of 86 days.The content of HSP70 was induced at the age of 35 days.There were no significant difference of HSP90 and HSP70 content between the chickens in the blank control and vaccine control group in the test process.HSP90 was mainly distributed in the cytoplasm of tumor cell and the content of HSP90 was significantly induced in the test process.HSP90 might be a marker to determine the process of tumor caused by MDV.

Key words: Marek’s disease, heat shock protein, histopathology, immunohistochemistry

中图分类号:

裴兰英，冯秀娟，李娟，李玉保，王守荣，朱育伟，吴伟胜. 马立克病病毒感染鸡心组织肿瘤病变与热休克蛋白表达的相关性分析[J]. 畜牧兽医学报, 2014, 45(12): 2013-2018.

PEI Lan-ying，FENG Xiu-juan，LI Juan，LI Yu-bao，WANG Shou-rong，ZHU Yu-wei，WU Wei-sheng. Relationship between Expression Level of HSPs and Heart Tissue Pathological Lesion of Chickens in Progress of Marek’s Disease[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2013-2018.

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马立克病病毒感染鸡心组织肿瘤病变与热休克蛋白表达的相关性分析

Relationship between Expression Level of HSPs and Heart Tissue Pathological Lesion of Chickens in Progress of Marek’s Disease

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