孕激素受体在雌性山羊颈前神经节的分布

doi:10.11843/j.issn.0366-6964.2014.04.022

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (4): 661-665.doi: 10.11843/j.issn.0366-6964.2014.04.022

孕激素受体在雌性山羊颈前神经节的分布

郭晓，李强，徐永平^*，金秀芳，陈文东，王志豪，董伟，范洁

（西北农林科技大学动物医学院，杨凌 712100）

收稿日期:2013-10-09 出版日期:2014-04-23 发布日期:2014-04-22
通讯作者: 徐永平，E-mail:xuyp717@nwsuaf.edu.cn
作者简介:郭晓（1987-）,女，河南省获嘉县人，硕士，主要从事基础兽医学研究，E-mail:guoxiao8768@163.com
基金资助:
陕西省农业推广项目（k332021102）；国家自然科学基金项目(31072184)

The Distribution of PR in Superior Cervical Ganglion of Female Goats

GUO Xiao， LI Qiang， XU Yong-ping^*， JIN Xiu-fang， CHEN Wen-dong，WANG Zhi-hao， DONG Wei， FAN Jie

(College of Veterinary Medicine，Northwest A﹠F University，Yangling 712100， China)

Received:2013-10-09 Online:2014-04-23 Published:2014-04-22

摘要/Abstract

摘要：

为研究雌性山羊颈前神经节(SCG)是否具备接受孕激素作用的条件，从而推测孕激素是否参与靶器官内分泌过程的神经调控，本试验采用免疫组织化学SP法研究雌性山羊SCG中孕激素受体（PR）的分布特点。结果显示在雌性山羊的SCG中，PR免疫反应阳性产物广泛分布，在神经元胞体、施万细胞、卫星细胞、神经纤维中均有不同程度的着色。其中神经元均为PR阳性，细胞膜和细胞质呈黄褐色，PR为中等阳性，而细胞核呈棕褐色为强阳性。另外施万细胞、支持细胞和神经纤维中也有淡黄色、弱阳性的PR阳性产物。图像分析表明，神经元胞体PR的相对表达量极显著（P＜0.01）高于其他非神经成分。以上结果表明雌性山羊颈前神经节是孕激素作用的主要靶器官之一，提示孕激素可能作用于SCG交感节后神经元，从而参与SCG支配的靶器官的功能活动，而SCG内神经元上的PR有可能作为一个节点协调孕激素对内脏器官的内分泌调节和自主神经对内脏器官的神经调节。

关键词: 孕激素受体, 颈前神经节, 免疫组化SP法, 雌山羊

Abstract:

In order to explore whether the superior cervical ganglion (SCG) of female goats was equipped with the conditions for the action of progesterone，thereby speculating that whether progesterone was involved in neural regulation of endocrine processes of target organs，we used immunohistochemical SP method to detect the distribution of progesterone receptor (PR) in SCG of female goat.The result showed that PR-immunoreactive-products were widely distributed in the SCG of female goat，and different levels of staining existed in neurons，Schwann cells，satellite cells and nerve fibers.All the neurons were PR positive，of which the cell membrane and cytoplasm was brown as moderate positive，and nuclei was brown as strong positive.Schwann cells，satellite and nerve fibers also had yellow，weak positive PR-positive-product.Image analysis showed that PR in the nerve cells was extremely higher (P＜0.01) than other non-nerve cells.The result proved that the neurons in the SCG were the one of main target cells of progesterone，implying that progesterone may act on the sympathetic postganglionic neurons of the SCG，and thus participates in the activities of the target organ dominated by the SCG，and PR in the SCG might act as a network node to coordinate the endocrine regulation of progesterone and neuroregulation of autonomic nerve on the activities of the target organ.

Key words: progesterone receptor, superior cervical ganglion, immunohistochemical SP method, female goat

中图分类号:

S852.1

郭晓，李强，徐永平，金秀芳，陈文东，王志豪，董伟，范洁. 孕激素受体在雌性山羊颈前神经节的分布[J]. 畜牧兽医学报, 2014, 45(4): 661-665.

GUO Xiao， LI Qiang， XU Yong-ping， JIN Xiu-fang， CHEN Wen-dong，WANG Zhi-hao， DONG Wei， FAN Jie. The Distribution of PR in Superior Cervical Ganglion of Female Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 661-665.

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孕激素受体在雌性山羊颈前神经节的分布

The Distribution of PR in Superior Cervical Ganglion of Female Goats

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