生长分化因子9及其受体在成年狗睾丸中的表达与定位

doi:10.11843/j.issn.0366-6964.2014.04.009

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (4): 566-571.doi: 10.11843/j.issn.0366-6964.2014.04.009

生长分化因子9及其受体在成年狗睾丸中的表达与定位

白瑞¹，赵鹂²，李栋²，于志慧¹，贺俊平^1*

（1.山西农业大学动物科技学院，太谷 030801； 2.揭阳爱维艾夫医院，揭阳 522051）

收稿日期:2013-11-27 出版日期:2014-04-23 发布日期:2014-04-22
通讯作者: 贺俊平，E-mail：dnhjp@163.com
作者简介:白瑞（1981-），男，山西太谷人，博士，讲师，主要从事羊驼毛色基因功能的研究，E-mail：chinabairui@163.com；赵鹂（1985-），女，山西阳泉人，硕士，主要从事生殖生物学、胚胎学研究，E-mail:414112175@qq.com；白瑞和赵鹂为并列第一作者
基金资助:
山西省科技攻关项目（20120311022-1）；山西农业大学科学人才引进基金(XB2008006)

Expression and Location of Growth Differentiation Factor 9 (GDF9) and Its Receptor in Adult Dog Testis

BAI Rui¹，ZHAO Li²，LI Dong²，YU Zhi-hui¹，HE Jun-ping^1*

(1.College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu 030801, China; 2.Jieyang IVF Hospital，Jieyang 522051, China)

Received:2013-11-27 Online:2014-04-23 Published:2014-04-22

摘要/Abstract

摘要：

本研究旨在探讨生长分化因子9（GDF9）及其受体在雄性成年狗睾丸中的定位与表达。采用RT-PCR、免疫组织化学法、免疫印迹法检测GDF9及其受体ALK5蛋白在成年狗睾丸中的表达与定位。GDF9蛋白在成年狗睾丸中的表达显示，GDF9阶段特异性地定位在成年狗睾丸生精上皮的圆形精细胞和粗线期精母细胞胞质。GDF9 I型膜受体ALK5蛋白在成年狗睾丸中的表达显示，ALK5主要定位在圆形精细胞。本研究显示，GDF9及其膜受体ALK5在成年狗睾丸中均有表达，提示，GDF9由圆形精细胞和粗线期精母细胞特异性分泌，通过旁分泌形式调控支持细胞间紧密连接及支持细胞与生殖细胞间的细胞连接，并以自分泌形式调控生殖细胞的生长与迁移。

关键词: 狗, 睾丸, 精子发生, 生长分化因子9, ALK5

Abstract:

The purposes of the study were to explore growth differentiation factor 9(GDF9) and its receptor position and expression in the adult dog testis.The expression and location of GDF9 and its receptor activin receptor-like kinase 5(ALK 5) in adult dog testis was detected by RT-PCR，immunohistochemistry and Western blot.The expression of protein GDF9 in testis of adult dogs can show that GDF9 is stage-specifically localized in the cytoplasm of round spermatids and pachytene spermatocytes of adult dog seminiferous epithelium.In particular，the expression of protein ALK5 in testis of adult dogs can show that ALK5 is localized in the cytoplasm of round spermatids.The study showed that GDF9 and its membrane receptor ALK5 were both expressed in adult dog testis.GDF9 is specifically secreted by the cytoplasm of round spermatids and pachytene spermatocytes.GDF9 regulates and sustains the connections between Sertoli cells，and between cells and germ cells by paracrine secretion.Also，GDF9 regulates the growth and migration of germ cells by autocrine secretion.

Key words: dog, testis, spermatogenesis growth, differentiation factor 9, activin receptor-like kinases 5

中图分类号:

S829.2.2

白瑞，赵鹂，李栋，于志慧，贺俊平. 生长分化因子9及其受体在成年狗睾丸中的表达与定位[J]. 畜牧兽医学报, 2014, 45(4): 566-571.

BAI Rui，ZHAO Li，LI Dong，YU Zhi-hui，HE Jun-ping. Expression and Location of Growth Differentiation Factor 9 (GDF9) and Its Receptor in Adult Dog Testis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 566-571.

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生长分化因子9及其受体在成年狗睾丸中的表达与定位

Expression and Location of Growth Differentiation Factor 9 (GDF9) and Its Receptor in Adult Dog Testis

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