猪MSTN基因的多态性和生长性状关联分析

doi:10.11843/j.issn.0366-6964.2013.07.011

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (7): 1063-1069.doi: 10.11843/j.issn.0366-6964.2013.07.011

猪MSTN基因的多态性和生长性状关联分析

刘晓琴^1,2，马喜山¹，唐中林^1*，周荣¹，杨述林¹，敖红¹，牟玉莲¹，李奎¹

（1.中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室，北京 100193; 2. 湖南农业大学动物科学技术学院，长沙 410128）

收稿日期:2012-11-05 出版日期:2013-07-23 发布日期:2013-07-29
通讯作者: 唐中林（1974-），副研究员，E-mail: zhonglinqy_99@sina.com
作者简介:刘晓琴（1988-），女，湖南怀化人，硕士生，主要从事动物遗传育种与繁殖方面研究，E-mail:liuxiaoqin112@163.com
基金资助:
转基因重大专项（2011ZX08009-001）;国家863项目（2011AA100300）;广西家畜遗传改良重点实验室培育基地开放课题（桂牧科研2012-27）

Polymorphism of MSTN Gene and Its Association with Growth Traits in Porcine

LIU Xiao-qin¹^，2, MA Xi-shan¹, TANG Zhong-lin^1*, ZHOU Rong¹, YANG Shu-lin¹,AO Hong¹, MU Yu-lian¹, LI Kui¹

(1. Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193，China； 2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China)

Received:2012-11-05 Online:2013-07-23 Published:2013-07-29
Supported by:

摘要/Abstract

摘要：

基于肌肉生长抑制素（Myostatin，MSTN）基因结构及功能研究报道，将其作为猪生长发育性状的候选基因进行多态性检测与关联分析，旨在为猪分子遗传标记提供依据。本研究以长白猪、大白猪、杜长大、通城猪、莱芜猪、五指山猪6个不同猪种基因组DNA为模板，通过克隆测序鉴定MSTN基因启动子区、第1内含子区、第2内含子区、第1外显子区、第2外显子区及3′UTR区SNPs位点；以长白猪和杜长大2个试验猪群为材料，利用基质辅助激光解吸飞行时间质谱法对MSTN基因进行SNP分型。建立最小二乘法分析模型，利用SAS软件分析数据。结果表明：在6个猪种76个个体中，共筛选出16个SNPs，其中有4个位于启动子区，5个位于第1内含子，7个位于第2内含子，在外显子1、外显子2和3′UTR区域并未检测到SNP位点。对MSTN基因的4个SNPs位点（P1、P3、P4、P5；其中P1、P3、P4位于启动子区，P5位于内含子1区）的生长性状关联分析显示，P4和P5 2个位点的多态性与猪生长性状显著相关（P<0.05）。P4和P5 2个位点具有作为分子标记辅助猪育种的潜在应用价值。

关键词: font-size: 10.5pt, mso-bidi-font-size: 11.0pt, mso-ascii-font-family: Calibri, mso-ascii-theme-font: minor-latin, mso-fareast-theme-font: minor-fareast, mso-hansi-font-family: Calibri, mso-hansi-theme-font: minor-latin, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">font-size: 10.5pt, mso-bidi-font-size: 11.0pt, mso-ascii-font-family: Calibri, mso-ascii-theme-font: minor-latin, mso-fareast-theme-font: minor-fareast, mso-hansi-font-family: Calibri, mso-hansi-theme-font: minor-latin, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">猪, MSTN, 多态性, 生长性状, 关联分析

Abstract:

Myostatin gene (MSTN) was regarded as a candidate gene for selection of porcine growth trait. In this study, the SNPs of MSTN gene had been detected and its association with porcine growth had been analyzed based on reports on its gene structure and function to put it as molecular genetic markers in porcine breeding. Using templates of genome DNA from 6 breeds including Landrace, Yorkshire, Duroc×(Landrace×Large White), Tongcheng pig, Wuzhishan pig, Laiwu pig, the SNPs were identified by cloning and sequencing of MSTN gene located in its promoter region, intron1, intron2, exon1, exon2 and 3′UTR. Matrix assisted laser desorption ionization time-of-flight mass spectrometry methed was used to genotype SNPs in MSTN gene in Landrace and Duroc×(Landrace×Large white) two experimental group and the data was assessdzed by SAS software. Total 16 SNPs were screened from 76 individuals of 6 breeds, of which 4 located in the promoter region, 5 located intron1, 7 located in intron2, while no SNPs was discovered in exon1, exon2 and its 3′UTR region. Association analysis of four SNPs (P1、P3 and P4 located in the promoter region; P5 located in intron1) in MSTN gene with growth traits indicated that P4 and P5 were significantly correlated (P<0.05) with growth traits. Both P4 and P5 have the potential application value as molecular marker in porcine breeding

Key words: font-size: 10.5pt, mso-bidi-font-size: 11.0pt, mso-ascii-theme-font: minor-latin, mso-fareast-theme-font: minor-fareast, mso-hansi-theme-font: minor-latin, mso-bidi-font-family: 'Times New Roman', mso-bidi-theme-font: minor-bidi, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang="EN-US">pig, MSNT, polymorphism, growth traits, association analysis

中图分类号:

S828.2

刘晓琴，马喜山，唐中林，周荣，杨述林，敖红，牟玉莲，李奎. 猪MSTN基因的多态性和生长性状关联分析[J]. 畜牧兽医学报, 2013, 44(7): 1063-1069.

LIU Xiao-qin, MA Xi-shan, TANG Zhong-lin, ZHOU Rong, YANG Shu-lin,AO Hong, MU Yu-lian, LI Kui. Polymorphism of MSTN Gene and Its Association with Growth Traits in Porcine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1063-1069.

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猪MSTN基因的多态性和生长性状关联分析

Polymorphism of MSTN Gene and Its Association with Growth Traits in Porcine

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