孕激素受体在雌性山羊腹腔肠系膜前神经节的分布

doi:10.11843/j.issn.0366-6964.2013.06.019

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (6): 960-964.doi: 10.11843/j.issn.0366-6964.2013.06.019

孕激素受体在雌性山羊腹腔肠系膜前神经节的分布

郭晓，王志豪，吴卫妮，徐永平^*，任妮，董伟，陈文东，范洁，金秀芳

（西北农林科技大学动物医学院，杨凌 712100）

收稿日期:2013-01-05 出版日期:2013-06-23 发布日期:2013-06-27
通讯作者: 徐永平, 副教授，E-mail: xuyp717@nwsuaf.edu.cn
作者简介:郭晓（1987-），女，河南省获嘉县人，硕士，主要从事基础兽医学的研究，E-mail: guoxiao8768@163.com，Tel：18706870121
基金资助:
陕西省农业推广项目（k332021102）；国家自然科学基金项目(31072184)

Distribution of Progesterone Receptor in Celiac Superior Mesenteric Ganglion of Female Goat

GUO Xiao, WANG Zhi-hao, WU Wei-ni, XU Yong-ping^*, REN Ni, DONG Wei,CHEN Wen-dong, FAN Jie, JIN Xiu-fang

(College of Veterinary Medicine,Northwest A & F University, Yangling 712100, China)

Received:2013-01-05 Online:2013-06-23 Published:2013-06-27

摘要/Abstract

摘要：

为了研究孕激素对胃肠道的内分泌调节和自主神经对胃肠道的神经调节两种途经之间的相互关系，作者采用免疫组织化学SP法，观察孕激素受体(PR)在成年雌性山羊腹腔肠系膜前神经节的分布特点。结果显示，雌山羊腹腔肠系膜前神经节中PR免疫阳性产物广泛存在，且主要分布于神经元中。神经元的细胞膜呈棕褐色，为强阳性，细胞质为强阳性或中等阳性。43.40%的神经元细胞核内核质呈黄褐色，核仁为清亮的空泡；56.60%的神经元核质不着色，核仁的PR为中等阳性。图像分析表明神经元的PR相对表达量与其他非神经结构相比差异性极显著（P＜0.01）。结果表明，腹腔肠系膜前神经节的交感节后神经元是孕激素作用的靶细胞，提示腹腔肠系膜前神经节上的PR可能是孕激素对胃肠道内分泌调节和自主神经对胃肠道的神经调节之间达到相互协调作用的网络节点。

关键词: 腹腔肠系膜前神经节, 孕激素受体, 免疫组织化学SP法, 雌山羊

Abstract:

In order to explore the relationship of regulatory pathways between progesterone’s endocrine regulation and autonomic nerve’s neuroregulation on gastrointestinal function, the distribution of progesterone receptor (PR) in celiac superior mesenteric ganglion of female goat was detected by immunohistochemical SP method. The results showed that PR immunoreactive products distributed widely in the celiac superior mesenteric ganglion of female goat, and mainly in nerve cells. The cell membrane of neurons was brown as strong positive, and cytoplasm was strong or moderate positive. 43.40% neurons’ karyoplasm was tawny, but the nucleolus was clear vacuoles; 56.60% neurons’ karyoplasm had no staining, but nucleolus was moderate positive. Image analysis showed that PR in the nerve cells was extremely significant compared with other non-nerve cells (P＜0.01). The result proved that the neurons in celiac superior mesenteric ganglion were the main target cells of progesterone, and PR in celiac superior mesenteric ganglion might be the network node to coordinate the endocrine regulation of progesterone and neuroregulation of autonomic nerve on gastrointestinal function.

Key words: celiac superior mesenteric ganglion, progesterone receptor, immunohistochemical SP method, female goat

中图分类号:

S852.16

郭晓，王志豪，吴卫妮，徐永平，任妮，董伟，陈文东，范洁，金秀芳. 孕激素受体在雌性山羊腹腔肠系膜前神经节的分布[J]. 畜牧兽医学报, 2013, 44(6): 960-964.

GUO Xiao, WANG Zhi-hao, WU Wei-ni, XU Yong-ping, REN Ni, DONG Wei,CHEN Wen-dong, FAN Jie, JIN Xiu-fang. Distribution of Progesterone Receptor in Celiac Superior Mesenteric Ganglion of Female Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 960-964.

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孕激素受体在雌性山羊腹腔肠系膜前神经节的分布

Distribution of Progesterone Receptor in Celiac Superior Mesenteric Ganglion of Female Goat

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