miR-200家族在西农萨能奶山羊乳腺组织中的表达分析

doi:10.11843/j.issn.0366-6964.2013.06.017

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (6): 944-951.doi: 10.11843/j.issn.0366-6964.2013.06.017

miR-200家族在西农萨能奶山羊乳腺组织中的表达分析

张犁苹，罗军^*，林先滋，朱江江，石恒波，史怀平，李君

（西北农林科技大学动物科技学院，杨凌 712100）

收稿日期:2012-12-20 出版日期:2013-06-23 发布日期:2013-06-27
通讯作者: 罗军，教授，E-mail: luojun1@yahoo.com
作者简介:张犁苹(1988-)，女，甘肃景泰县人，硕士生，主要从事生物技术与动物育种研究，E-mail: aoxue0811@yahoo.cn
基金资助:
公益性行业（农业）科研专项（201103038）；陕西省重大科技创新项目（2009ZKC07-01-1）

Expression Profiles of miR-200 Family in Mammary Gland of Xinong SaanenDairy Goat

ZHANG Li-ping, LUO Jun^*, LIN Xian-zi, ZHU Jiang-jiang, SHI Heng-bo, SHI Huai-ping, LI Jun

(College of Animal Science and Technology, Northwest A&F University,Yangling 712100, China)

Received:2012-12-20 Online:2013-06-23 Published:2013-06-27

摘要/Abstract

摘要：

笔者拟获得山羊miR-200家族乳腺组织表达谱，研究miR-200家族成员之间表达相关性及miR-200家族成员与山羊乳成分的相关性。通过定量PCR，检测miR-200家族在西农萨能奶山羊泌乳中期和干奶期乳腺组织中的表达量；利用Pearson相关系数分析miR-200家族不同成员表达量之间的相关性，同时分析miR-200家族成员与山羊乳成分之间的相关关系；利用miRanda和TargetScan 6.2软件分析、 RNAhybrid和PITA软件预测miR-200 家族的靶基因。结果表明，miR-200家族在奶山羊泌乳中期的乳腺组织中的表达量极显著高于干奶期（P <0.01）；miR-200a与miR-141之间的表达量显著相关（P <0.05），miR-200b与miR-200c之间的表达量极显著相关（P <0.01）；泌乳中期奶山羊乳腺组织中miR-200c的表达量与乳蛋白、非脂固形物（SNF）、冰点（FPD）的含量显著相关（P<0.05）；经软件分析及预测发现，miR-200a/miR-141靶向作用于在乳脂合成中起关键作用的基因STAT4。miR-200家族可能对山羊乳蛋白及乳脂合成具有一定的调控作用。

关键词: miR-200家族, 西农萨能奶山羊, 乳腺组织表达, 靶基因

Abstract:

The aim of the study was to establish the expression profiles of miR-200 family in mammary gland tissues of Xinong Saanen dairy goats, and to analyze the correlation between miR-200 family and milk composition. Real-Time qPCR was used for detecting the expression of miR-200 family in mammary gland during mid-lactation and dry period. Pearson’s correlation coefficient analysis was used to determine the correlations of different miR-200 family members and goat milk composition. MiRanda, TargetScan 6.2, RNAhybrid, and PITA software were used for predicting the target genes of miR-200 family. Results showed that the expression levels of all miR-200 family members during mid-lactation were significantly higher than that during dry period (P < 0.01). Correlation analysis showed the significant correlations between miR-200a and miR-141 (P<0.05), and miR-200b and miR-200c (P<0.01). Furthermore, the expression levels of miR-200c significantly correlated with the contents of goat milk protein, solids-not-fat (SNF), and Freezing point depression (FPD) (P<0.05). We also found STAT4, a key regulator for milk fat synthesis, was a predicted target genes of miR-200a and miR-141. We concluded that miR-200 family might regulate milk protein and fatty acid synthesis in lactating goats.

Key words: miR-200 family, Xinong Saanen dairy goat, expression in mammary gland tissues, target gene

中图分类号:

S827
S813.3

张犁苹，罗军，林先滋，朱江江，石恒波，史怀平，李君. miR-200家族在西农萨能奶山羊乳腺组织中的表达分析[J]. 畜牧兽医学报, 2013, 44(6): 944-951.

ZHANG Li-ping, LUO Jun, LIN Xian-zi, ZHU Jiang-jiang, SHI Heng-bo, SHI Huai-ping, LI Jun. Expression Profiles of miR-200 Family in Mammary Gland of Xinong SaanenDairy Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 944-951.

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miR-200家族在西农萨能奶山羊乳腺组织中的表达分析

Expression Profiles of miR-200 Family in Mammary Gland of Xinong SaanenDairy Goat

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