猪肺炎支原体不同蛋白抗原检测疫苗诱导IgG产生规律的研究

doi:10.11843/j.issn.0366-6964.2013.06.014

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (6): 925-930.doi: 10.11843/j.issn.0366-6964.2013.06.014

猪肺炎支原体不同蛋白抗原检测疫苗诱导IgG产生规律的研究

杜改梅^1,2，刘茂军¹，韦艳娜¹，甘源¹，马庆红¹，邵国青^1*

（1. 江苏省农业科学院兽医研究所农业部兽用生物制品工程技术重点实验室国家兽用生物制品工程技术研究中心，南京 210014；2.金陵科技学院动物科学与技术学院,南京 210038）

收稿日期:2012-12-10 出版日期:2013-06-23 发布日期:2013-06-27
通讯作者: 邵国青，男，博士，研究员，主要从事猪病防控研究，E-mail：84391973@163.com
作者简介:杜改梅（1976-），女，副教授，博士，主要从事猪传染病防控研究，E-mail：gaimeidu@163.com
基金资助:
国家自然科学基金项目（31103116）;江苏省博士后基金项目（6511201）

Detection of IgG Antibodies Regularity Induced by Vaccine Using Different Mycoplasma hyopneumoniae Antigen Proteins

DU Gai-mei^1,2, LIU Mao-jun¹, WEI Yan-na¹, GAN Yuan¹, MA Qing-hong¹, SHAO Guo-qing^1*

(1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Enginerring and Technology of Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; 2. Department of Animal Science and Technology, Jinling Technology Institution, Nanjing 210038, China)

Received:2012-12-10 Online:2013-06-23 Published:2013-06-27

摘要/Abstract

摘要：

为了筛选出特异性好和敏感性高的ELISA用猪肺炎支原体抗原蛋白，作者分别将猪肺炎支原体灭活疫苗和168株菌活疫苗分别经肌肉和肺内注射途径免疫猪，免疫后7、15、30和56 d采集分离血清。以P97R1、P36、P46、DnaK单个蛋白和混合蛋白为猪支原体肺炎血清抗体ELISA用特异性蛋白抗原，与猪肺炎支原体IDEXX抗体检测试剂盒比较检测免疫后抗体的发生规律，并对检测结果进行对比。结果表明，P36和P97R1能够检测出比较高的母源抗体水平；DnaK和P46检测的抗体滴度变化趋势相一致，且检测的抗体高滴度维持时间较长；与其它抗原相比，混和蛋白在56 d时检测到较高的抗体水平。混合抗原的检测结果与IDEXX具有较高的符合率，但检测敏感性比IDEXX高，且较IDEXX符合抗体变化规律。结果表明混合蛋白具有较高的检测敏感性，可以更加准确地监测该病的感染或免疫状态。

关键词: 猪肺炎支原体, IgG, 疫苗, P97R1, P36, P46, DnaK

Abstract:

The present study was undertaken to screen antigen proteins with high specificity and sensitivity in Mycoplasma hyopneumoniae. Swine were vaccinated intramuscularly and intrapulmonary with Mycoplasma hyopneumoniae inactivated vaccine and attenuated M. hyopneumoniae 168 strain live vaccine. Serum samples were collected on 7, 15, 30 and 56 d post immunization. Antibody occurrence was detected using each and all of P97R1, P36, P46, DnaK proteins as specificity antigenic protein, respectively. And the further compare detection test was investigated. The results showed that P36 and P97R1 proteins based ELISA could detect relatively high maternal antibody levels; Antibody occurrence detected by DanK protein based ELISA was in accordance with that of P46 protein, and both had long-term high antibody titers. Compared with other proteins, mix proteins antigen based ELISA could detect high antibody titers on 56 d after immunization. Mix proteins based ELISA was satisfactory and high complicated with IDEXX antibody detection kit. But it has higher sensitivity than IDEXX antibody detection kit. And antibody occurrence detected by mixed proteins based ELISA was more in accordance with antibody occurrence regularity than IDEXX antibody detection kit. The results showed that the mix proteins based ELISA has high detection sensitivity, which indicated that this method can more accurately monitoring the infection status and animal immune status.

Key words: Mycoplasma hyopneumoniae, IgG, vaccine, P97R1, P36, P46, DnaK

中图分类号:

S852.62

杜改梅，刘茂军，韦艳娜，甘源，马庆红，邵国青. 猪肺炎支原体不同蛋白抗原检测疫苗诱导IgG产生规律的研究[J]. 畜牧兽医学报, 2013, 44(6): 925-930.

DU Gai-mei, LIU Mao-jun, WEI Yan-na, GAN Yuan, MA Qing-hong, SHAO Guo-qing. Detection of IgG Antibodies Regularity Induced by Vaccine Using Different Mycoplasma hyopneumoniae Antigen Proteins[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 925-930.

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猪肺炎支原体不同蛋白抗原检测疫苗诱导IgG产生规律的研究

Detection of IgG Antibodies Regularity Induced by Vaccine Using Different Mycoplasma hyopneumoniae Antigen Proteins

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