基于纳米材料的电化学免疫传感器检测H5N1亚型禽流感病毒的研究

doi:10.11843/j.issn.0366-6964.2013.06.012

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (6): 911-918.doi: 10.11843/j.issn.0366-6964.2013.06.012

基于纳米材料的电化学免疫传感器检测H5N1亚型禽流感病毒的研究

黄娇玲，谢芝勋^*，罗思思，谢志勤，谢丽基，刘加波，庞耀珊，范晴

（广西壮族自治区兽医研究所广西畜禽疫苗新技术重点实验室，南宁 530001）

收稿日期:2012-12-26 出版日期:2013-06-23 发布日期:2013-06-27
通讯作者: 谢芝勋，二级研究员，E-mail：xiezhixun@126.com
作者简介:黄娇玲(1987-)，女，壮族，广西大新县人，硕士，主要从事电化学生物传感器的研究，E-mail: 331590778@qq.com，Tel：0771-3120371
基金资助:
广西特聘专家专项经费（2011B020）；广西科技攻关重大专项（1222003-2-4）

A Electrochemical Immunosensor Detection of Avian Influenza Virus (H5N1) Based on Nano Materials

HUANG Jiao-ling, XIE Zhi-xun^*, LUO Si-si, XIE Zhi-qin, XIE Li-ji, LIU Jia-bo, PANG Yao-shan, FAN Qing

(Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi VeterinaryResearch Institute, Nanning 530001, China)

Received:2012-12-26 Online:2013-06-23 Published:2013-06-27

摘要/Abstract

摘要：

利用氧化石墨烯(GO)负载H5N1亚型禽流感病毒多克隆抗体(PAb-H5N1)及牛血清白蛋白(BSA) 作为信号放大材料，构建一种新型电化学免疫传感器用于检测H5N1亚型禽流感病毒。结果表明：以PAb-H5N1-GO-BSA纳米复合物作为信号放大材料构建的电化学免疫传感器的灵敏度比不用此纳米复合物作为信号放大的高256倍。以PAb-H5N1-GO-BSA纳米复合物作为信号放大材料构建的电化学免疫传感器对H5N1亚型禽流感病毒的检测限为2^-15HA unit·50 μL^-1，检测线性范围为2^-15~2^-8 HA unit·50 μL^-1。此传感器特异性好，灵敏度高，在病原微生物快速检测领域具有良好的应用前景。

关键词: 氧化石墨烯, 电化学免疫传感器, 金纳米粒子, H5N1亚型禽流感病毒

Abstract:

A novel electrochemical immunosensor for sensitive detection of avian influenza virus (AIV) H5N1 was described. A graphene oxide (GO) carrying H5N1-polychonal antibodies (PAb-H5N1) and Bovine serum albumin (BSA) were used as signal amplification materials. On the basis of the signal amplification strategy of PAb-H5N1-GO-BSA nanocomposite, the developed immunosensor showed a 256-fold increase in detection limit compared to the immunosensor without PAb-H5N1-GO-BSA nanocomposite amplification. The developed method could detect 2^-15 HA unit·50 μL^-1 H5N1 with a linear calibration range from 2^-15 to 2^-8 HA unit·50 μL^-1. This immunosensor has a good specificity and high sensitivity, and it is promising for rapid detection of pathogenic microorganisms.

Key words: graphene oxide, electrochemical immunoassay, gold nanoparticles, avian influenza virus H5N1

中图分类号:

黄娇玲，谢芝勋，罗思思，谢志勤，谢丽基，刘加波，庞耀珊，范晴. 基于纳米材料的电化学免疫传感器检测H5N1亚型禽流感病毒的研究[J]. 畜牧兽医学报, 2013, 44(6): 911-918.

HUANG Jiao-ling, XIE Zhi-xun, LUO Si-si, XIE Zhi-qin, XIE Li-ji, LIU Jia-bo, PANG Yao-shan, FAN Qing. A Electrochemical Immunosensor Detection of Avian Influenza Virus (H5N1) Based on Nano Materials[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 911-918.

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基于纳米材料的电化学免疫传感器检测H5N1亚型禽流感病毒的研究

A Electrochemical Immunosensor Detection of Avian Influenza Virus (H5N1) Based on Nano Materials

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