FSH和17β-雌二醇联合作用对Cyclin D1 mRNA和Cyclin E1 mRNA表达的影响

doi:10.11843/j.issn.0366-6964.2013.06.005

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (6): 858-865.doi: 10.11843/j.issn.0366-6964.2013.06.005

FSH和17β-雌二醇联合作用对Cyclin D1 mRNA和Cyclin E1 mRNA表达的影响

王怡，张姣姣，汪勇，张会琼，孙思，王鲜忠^*，张家骅

（西南大学动物科技学院, 重庆市牧草与草食家畜重点实验室，重庆 400716）

收稿日期:2012-12-03 出版日期:2013-06-23 发布日期:2013-06-27
通讯作者: 王鲜忠，教授，博士，E-mail：xianzhang_wang@yahoo.com.cn
作者简介:王怡(1989-），女，四川三台人，硕士生，主要从事动物生殖生理的研究，E-mail：xinan2009@126.com
基金资助:
国家自然科学基金项目(31072183)

Effect of Combination of FSH and 17β-estradiol on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell

WANG Yi, ZHANG Jiao-jiao, WANG Yong, ZHANG Hui-qiong, SUN Si,WANG Xian-zhong^*, ZHANG Jia-hua

(Chongqing Key Laboratory of Forage & Herbivore, College of Animal Science and Technology, Southwest University，Chongqing 400716, China)

Received:2012-12-03 Online:2013-06-23 Published:2013-06-27

摘要/Abstract

摘要：

为了确定FSH和雌激素联合作用对培养条件下未成熟仔猪睾丸支持细胞中Cyclin D1 mRNA和Cyclin E1 mRNA表达的影响。以培养的仔猪睾丸支持细胞为研究材料，通过添加各种信号通路的抑制剂，应用实时荧光定量PCR检测Cyclin D1 mRNA和Cyclin E1 mRNA的相对表达量。FSH(50 ng·mL^-1)和17β-雌二醇 (10^-9 mol·L^-1)联合作用时对Cyclin D1 mRNA和Cyclin E1 mRNA表达的影响与FSH或17β-雌二醇 (10^-9mol·L^-1)单独作用相比无显著影响(P>0.05)；环磷酸腺苷抑制剂（Rp-cAMP）、L-Ca²⁺离子通道抑制剂（Verapamil）和ERK1/2抑制剂（U0126）单独作用时对Cyclin D1 mRNA和Cyclin E1 mRNA表达与空白对照组相比无显著影响(P>0.05)，但以剂量依赖的方式抑制了FSH(50 ng·mL^-1)与17β-雌二醇(10^-9 mol·L^-1) 联合作用对Cyclin D1 mRNA和Cyclin E1 mRNA的表达(P<0.05)。FSH和17β-雌二醇联合作用时通过cAMP、Ca²⁺内流和ERK1/2途径参与了FSH和17β-雌二醇对Cyclin D1 mRNA和Cyclin E1 mRNA在细胞中的表达。

关键词: FSH, 17β-雌二醇, 睾丸支持细胞, 细胞周期蛋白D1, 细胞周期蛋白E1, 细胞外信号调节的蛋白激酶1/2

Abstract:

The objective of the study was to identify whether the combination of FSH and 17-beta-estradiol regulated the mRNA expression of Cyclin D1 and Cyclin E1 in the cultured immature boar Sertoli cells. Cultured immature boar Sertoli cells were treated with the combination of 17-beta-estradiol (10^-9 mol·L^-1) and FSH (50 ng·mL^-1) and added a variety of different signaling pathway inhibitors. And Real-time PCR was applied to detect the mRNA expression of Cyclin D1 and Cyclin E1. Compared to FSH (50 ng·mL^-1) or 17-beta-estradiol (10^-9mol·L^-1) alone, the combination of FSH and 17-beta-estradiol had no significant effect on the mRNA expression of Cyclin D1 mRNA and Cyclin E1(P>0.05). In addition, Rp-cAMP (cAMP inhibitor), Verapamil (L-type Ca²⁺ ionic channel inhibitor) or U0126 (ERK1/2 inhibitor) alone had no significant effect on the mRNA expression of Cyclin D1 and Cyclin E1 in comparison to the control group (no FSH or17-beta-estradiol) (P>0.05). However, three inhibitors could reduce the mRNA expression of Cyclin D1 and Cyclin E1 in a dose-dependent way (P<0.05, for all) when compared to the combined FSH (50 ng·mL^-1) and 17-beta-estradiol (10^-9 mol·L^-1). cAMP, Ca²⁺and ERK1/2 were involved in the effect of the combination of FSH and 17-beta-estradiol regulating the mRNA expression of Cyclin D1 and Cyclin E1.

Key words: FSH, 17-beta-estradiol, Sertoli cell, Cyclin D1, Cyclin E1, extracellular signal-regulated kinase 1/2

中图分类号:

S828
S814.1

王怡，张姣姣，汪勇，张会琼，孙思，王鲜忠，张家骅. FSH和17β-雌二醇联合作用对Cyclin D1 mRNA和Cyclin E1 mRNA表达的影响[J]. 畜牧兽医学报, 2013, 44(6): 858-865.

WANG Yi, ZHANG Jiao-jiao, WANG Yong, ZHANG Hui-qiong, SUN Si,WANG Xian-zhong, ZHANG Jia-hua. Effect of Combination of FSH and 17β-estradiol on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 858-865.

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FSH和17β-雌二醇联合作用对Cyclin D1 mRNA和Cyclin E1 mRNA表达的影响

Effect of Combination of FSH and 17β-estradiol on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell

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