牛消化道各段Ⅰ型肽载体（bPepTI）mRNA表达量的比较研究

摘要/Abstract

摘要：

旨在比较研究牛消化道各段牛Ⅰ型肽载体(bPepTI) mRNA的表达。本研究分别用比较Ct和标准曲线2种相对定量方法和1种绝对定量方法比较牛各段消化道上皮黏膜I型肽载体mRNA的表达量，以了解小肽在牛消化道的主要吸收部位。比较Ct法测定牛各段消化道表达量无显著统计差异（P>0.05），数值上由大到小的顺序为：空肠、回肠、盲肠、十二指肠、瘤胃、结肠、皱胃、瓣胃、网胃；标准曲线法结果表明，牛消化道各段bPepTI mRNA表达量差异显著（P<0.05），其中空肠的表达量最高，显著高于回肠（P<0.05），回肠表达量与消化道其余组织差异显著（P<0.05），其余各组织间表达量差异不显著（P>0.05）；绝对定量检测到牛消化道各部位bPepTI mRNA表达量总体差异显著（P<0.05），mRNA拷贝数由高到低依次为空肠、瓣胃、网胃、瘤胃、盲肠、十二指肠、回肠、皱胃、结肠，空肠的表达量显著高于瓣胃（P<0.05），盲肠、十二指肠、回肠、皱胃之间表达量差异不显著（P>0.05）。综合本研究结果，标准曲线法和绝对定量测定牛消化道I型肽载体mRNA表达量更为准确，在牛整段消化道中I型肽载体mRNA在空肠、回肠、瓣胃、瘤胃都有高表达，应是小肽吸收的主要部位。

关键词: 牛Ⅰ型肽载体, 相对定量法, 绝对定量发, 表达

Abstract:

The aim of the study was to investigate the expression of bovine peptide transporter I(bPepTI) mRNA in bovine Gastrointestinal(GI) tract. Relative and absolute quantitative mRNA methods were used to detect the expression of bPepTI mRNA in different parts of bovine gastrointestinal tract and to investigate its main absorption sites in the present study. Both the -ΔC_tand standard curve methods were used in relative quantification. The results of -ΔC_t method revealed no significant difference among different parts of bovine GI tract (P>0.05). Numerically, mRNA expression of bPepTI decreased in order of reticulum < omasum < abomasums < colon < rumen < duodenum< caecum< ileum< jejunum. The results of standard curve method were significantly different among different parts of bovine GI tract (P<0.05). mRNA expression of bPepTI in jejunum was significantly higher than that in ileum (P<0.05); the expression of bPepTI in ileum was significantly higher than that in other parts (P<0.05); and there were no significant differences among the other parts of bovine GI tract (P>0.05). The data from absolute quantification showed that mRNA copies of bPepTI were different in different parts of bovine tract (P<0.05), and the order from the highest to the lowest mRNA copies of bPepTI was jejunum,omasum, reticulum, rumen, caecum, duodenum, ileum, abomasums and colon. mRNA copies of bPepTI in jejunum was significantly higher than that in omasum (P<0.05), and there was no significant differences among caecum, duodenum, ileum, abomasum (P>0.05). In conclusion, results of standard curve and absolute quantification method were more believable,and jejunum, ileum, omasum and rumen may be the major absorption parts of small peptides along bovine GI tract, in which there were high mRNA expression of bPepTI.

Key words: bovine peptide transporter I, relative quantitative method, absolute quantitative method, expression

中图分类号:

S823
S813.3

刘桂梅，林雪彦，苏鹏程，王云，王中华. 牛消化道各段Ⅰ型肽载体（bPepTI）mRNA表达量的比较研究[J]. 畜牧兽医学报.

LIU Gui-mei, LIN Xue-yan, SU Peng-cheng, WANG Yun, WANG Zhong-hua. mRNA Expression of Peptide Transporter I in Different Parts of Bovine Gastrointestinal Tract[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA.

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