子宫内膜基质金属蛋白酶2、9 mRNA表达与奶牛子宫内膜炎关系的研究

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (5): 810-815.

子宫内膜基质金属蛋白酶2、9 mRNA表达与奶牛子宫内膜炎关系的研究

王国卿1,2，王洪海3，张乃生2

1.东北大学生物技术研究所，沈阳 110004； 2. 吉林大学畜牧兽医学院，长春 130062；3.大庆市畜牧兽医局，大庆 163311

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-05-28 发布日期:2012-05-28
通讯作者: 王国卿

The Relationship between MMP2, MMP9 mRNA of Bovine Endometrium and Endometritis

WANG Guoqing1,2, WANG Honghai3, ZHANG Naisheng2

1. Biotechnology Research Institute, Northeast University, Shenyang 110004, China; 2.College of Animal Husbandry and Veterinary Science, Jilin University, Changchun 1300622, China; 3. Pasturage Veterinary Bureau of Daqing City, Daqing 163311, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-05-28 Published:2012-05-28
Contact: WANG Guoqing

摘要/Abstract

摘要： 为探讨基质金属蛋白酶（MMPs）2、MMP9在奶牛子宫内膜炎中的作用机制，选用产后6～10 d健康及患急性化脓性子宫内膜炎的中国荷斯坦奶牛各10头，分别为对照组和试验组，通过ELISA检测PGF2a、孕酮浓度；免疫组化检测子宫内膜Ⅰ型胶原、Ⅳ型胶原的表达；SYBR Green I荧光定量PCR检测子宫内膜MMP2和MMP9 mRNA表达。结果表明：试验组PGF2a浓度极显著低于对照组（P<0.01），孕酮含量显著高于对照组（P<0.05），PGF2a与孕酮浓度呈显著负相关（r=0.893）；试验组子宫内膜中Ⅰ型胶原、Ⅳ型胶原的表达量均高于对照组，差异极显著(P<0.01)；MMP2和MMP9 mRNA水平则显著低于对照组（P<0.01）。结果提示：产后子宫内膜炎病牛子宫内膜MMP2、MMP9低表达，导致Ⅰ型和Ⅳ型胶原降解受阻，使细胞外基质为子宫内膜细胞提供的刺激信号不足，子宫分泌PGF2a量少，黄体消退障碍，血清孕酮含量高；说明子宫内膜MMP2、MMP9 mRNA表达对产后子宫内膜炎的发生和发展起促进作用。

关键词: 奶牛, 子宫内膜炎, MMPs, 荧光定量PCR, 胶原

Abstract: The study was conducted to investigate the role of MMP2 and MMP9 in cow endometritis. Healthy and suffering from acute purulent endometritis Chinese Holstein cows were selected as control and experimental group respectively with 10 in each group and all of the cows were 610 days postpartum. The concentration of PGF2a, Progesterone was detected by ELISA. CollagenⅠand Ⅳ in endometrium were checked by Immunohistochemistry. MMP2 and MMP9 expression were examined using realtime PCR in the endometrium. The results showed that the concentration of PGF2a in experimental group was lower than that in control group (P<0.01). The experimental group progesterone was significantly higher than the control group. The expression level of CollagenⅠand Ⅳ in endometristis was higher than the control group (P<0.01). The MMP2 and MMP9 mRNA expression in endometristis were lower than the control group (P<0.01). Conclusion indicated that the expression of MMP2 and MMP9 mRNA in cow endometristis reduced, CollagenⅠand Ⅳ degradation were blocked and endometrial cell was unable to secrete PGF2a, making the concentration of progesterone increase in blood serum. MMP2 and MMP9 mRNA influenced postpartum endometrial function and encouraged the development of postpartum endometritis.

Key words: cow, endometritis, MMPs, realtime PCR, collagen

王国卿;王洪海;张乃生. 子宫内膜基质金属蛋白酶2、9 mRNA表达与奶牛子宫内膜炎关系的研究[J]. 畜牧兽医学报, 2012, 43(5): 810-815.

WANG Guoqing;WANG Honghai;ZHANG Naisheng. The Relationship between MMP2, MMP9 mRNA of Bovine Endometrium and Endometritis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(5): 810-815.

[1]	王素华, 帅江冰, 李舟, 袁淑辉, 吴绍强, 吕继洲, 赵治国. 炭疽杆菌双重荧光定量PCR检测技术的建立和应用[J]. 畜牧兽医学报, 2019, 50(8): 1658-1665.
[2]	王素华, 帅江冰, 李舟, 袁淑辉, 吴绍强, 吕继洲, 赵治国. 炭疽杆菌双重荧光定量PCR检测技术的建立和应用[J]. 畜牧兽医学报, 2019, 50(8): 1658-1665.
[3]	刘江静, 陈晓丽, 田宏志, 路永强, 王栋. 返情母牛活动量影响因素分析[J]. 畜牧兽医学报, 2019, 50(7): 1405-1411.
[4]	刘江静, 陈晓丽, 田宏志, 路永强, 王栋. 返情母牛活动量影响因素分析[J]. 畜牧兽医学报, 2019, 50(7): 1405-1411.
[5]	刘豪丽, 滕蔓, 李会珍, 余祖华, 刘金玲, 丁轲, 张改平, 罗俊. 马立克病病毒编码的miR-M11-5p宿主靶基因的筛选与鉴定[J]. 畜牧兽医学报, 2019, 50(5): 1026-1038.
[6]	王玥琳, 郭紫晶, 岳华, 汤承. 部分地区奶牛腹泻粪便样中诺如病毒的检测和演化分析[J]. 畜牧兽医学报, 2019, 50(5): 1048-1055.
[7]	刘豪丽, 滕蔓, 李会珍, 余祖华, 刘金玲, 丁轲, 张改平, 罗俊. 马立克病病毒编码的miR-M11-5p宿主靶基因的筛选与鉴定[J]. 畜牧兽医学报, 2019, 50(5): 1026-1038.
[8]	王玥琳, 郭紫晶, 岳华, 汤承. 部分地区奶牛腹泻粪便样中诺如病毒的检测和演化分析[J]. 畜牧兽医学报, 2019, 50(5): 1048-1055.
[9]	吕天星, 李松建, 郝永清. 鉴别显色培养基在奶牛乳房炎病原菌快速鉴定中的应用[J]. 畜牧兽医学报, 2019, 50(4): 851-860.
[10]	王波, 刁志成, 马勇, 李发弟, 曲扬华, 徐晨晨, 卢晓楠, 罗海玲. 日粮添加油脂和出生类型对羔羊肌肉胶原蛋白特性及相关基因表达的影响[J]. 畜牧兽医学报, 2019, 50(4): 771-780.
[11]	王波, 刁志成, 马勇, 李发弟, 曲扬华, 徐晨晨, 卢晓楠, 罗海玲. 日粮添加油脂和出生类型对羔羊肌肉胶原蛋白特性及相关基因表达的影响[J]. 畜牧兽医学报, 2019, 50(4): 771-780.
[12]	吕天星, 李松建, 郝永清. 鉴别显色培养基在奶牛乳房炎病原菌快速鉴定中的应用[J]. 畜牧兽医学报, 2019, 50(4): 851-860.
[13]	邢智洋, 张梦璐, 张菡, 王月影, 韩立强, 高腾云, 杨国宇. 沉默SCAP基因对奶牛乳腺上皮细胞脂滴的影响[J]. 畜牧兽医学报, 2019, 50(3): 507-516.
[14]	邢智洋, 张梦璐, 张菡, 王月影, 韩立强, 高腾云, 杨国宇. 沉默SCAP基因对奶牛乳腺上皮细胞脂滴的影响[J]. 畜牧兽医学报, 2019, 50(3): 507-516.
[15]	李锐, 高海侠, 徐军, 王瑞, 王文静, 张瑞良, 赵霞, 李琳. RNA-Seq分析baeSR和acrB对鼠伤寒沙门菌相关毒力基因表达的影响[J]. 畜牧兽医学报, 2019, 50(11): 2318-2325.