奶牛围产期外周血T淋巴细胞亚群动态及细胞因子IL-2、IL-4基因转录水平分析

畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (11): 1615-1619.

奶牛围产期外周血T淋巴细胞亚群动态及细胞因子IL-2、IL-4基因转录水平分析

崔群维1，郭宁1，周璇1，王振云1，王雅春2*，王根林1*

1．南京农业大学动物科技学院，南京210095； 2. 中国农业大学动物科技学院，北京 100193

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-11-25 发布日期:2011-11-25
通讯作者: 王雅春2*，王根林1

Analysis on the Dynamic Changes of Lymphocyte Subsets and Genetic Transcription of Cytokine IL-2, IL-4 in Peripheral Blood of Dairy Cows during Perpartum

CUI Qunwei1, GUO Ning1, ZHOU Xuan1, WANG Zhenyun1, WANG Yachun2*, WANG Genlin1*

1．College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2．College of Animal Science and Technology, China Agricultural University,Beijing 100193, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25
Contact: WANG Yachun2*, WANG Genlin1*

摘要/Abstract

摘要： 通过检测奶牛CD3+、CD4+和CD8+淋巴细胞亚群的动态及细胞因子 IL2、IL4在分娩前后21 d内的基因转录水平的变化，以探讨此阶段奶牛免疫机能受损的机理。应用流式细胞术检测CD3+、CD4+、CD8+淋巴细胞亚群的动态变化及实时荧光定量检测细胞因子IL2、IL4 mRNA的表达量。结果表明：血液中CD3+淋巴细胞的比例在产前21 d内占（50.8±4.61）%，产后14 d内降低到（39.21±4.98）%，两者差异极显著（P<0.01）；CD4+淋巴细胞在产前21 d内保持较高水平，达（21.38±0.65）%，产后21 d内维持在（6.89±0.32）%的较低水平，且与产前差异显著 (P<0.05)；CD8+淋巴细胞的比例在产犊前逐渐升高，到产犊时达到最高水平（16.90±2.02）%，差异显著（P<0.05）；CD4+/CD8+淋巴细胞的比率在产前>1，产后<1。IL2 mRNA的表达量在产后0~14 d内下调，之后上升。IL4 mRNA的表达量分娩时期达到最高，随后下降，至第7天降到最低。奶牛在围产期阶段尤其是分娩前后 14 d内机体处于免疫抑制状态，可能是由于自身的CD3+、CD4+和CD8+T淋巴细胞的亚群的数量和比例发生改变及细胞因子IL2、IL4 mRNA表达量下调引起的。

关键词: 淋巴细胞亚群, 细胞因子, 免疫抑制, 实时荧光定量

Abstract: To reveal the mechanism of impaired immune function in perpartum period, the dynamic changes of CD3+, CD4+ and CD8+ in lymphocyte subsets as well as gene transcription level of cytokine IL2 and IL4 in peripheral blood of dairy cows during 21 days before calving to 21 days after it were investigated in the present study. The dynamic changes of Lymphocyte cells CD3+, CD4+, CD8+ and the mRNA expression of cytokine IL2, IL4 in peripheral blood were determined by flow cytometry and RTPCR respectively. The results showed that significant difference was found in the proportion of CD3+ in peripheral blood 21 days before calving (50.8±4.61)% and 14 days after caving (39.21±4.98)%, and also was found in the proportion of CD4+ in blood before (21.38±0.65)% and after (6.89±0.32)% calving. The proportion of CD8+ increased to the highest level (16.9±2.02)% during calving (P<0.05). The ratio of CD4+/CD8+ was >1 before calving, and <1 after calving. The mRNA expression of IL2 was the lowest (P<0.05) during 014 days after calving, then gradually increased. The mRNA expression of IL4 increased to the peak during calving, and declined to the lowest level on the 7th day (P<0.01). It was suggested that the immune function of dairy cows was impaired within 21 days before and after calving, especially during the 014 days after caving. This might be caused by the quantity and ratio changes of lymphocyte subsets (CD3+, CD4+and CD8+) and the downregulation of the cytokine IL2, IL4 during this critical period.

Key words: lymphocyte subsets, cytokine, immune function impaired, RT-PCR

崔群维;郭宁;周璇;王振云;王雅春;王根林. 奶牛围产期外周血T淋巴细胞亚群动态及细胞因子IL-2、IL-4基因转录水平分析[J]. 畜牧兽医学报, 2011, 42(11): 1615-1619.

CUI Qunwei;GUO Ning;ZHOU Xuan;WANG Zhenyun;WANG Yachun;WANG Genlin . Analysis on the Dynamic Changes of Lymphocyte Subsets and Genetic Transcription of Cytokine IL-2, IL-4 in Peripheral Blood of Dairy Cows during Perpartum[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(11): 1615-1619.

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