关键词: 替来他明, c-fos基因, 中枢神经系统

Abstract: To investigate the distribution of c-fos oncogene expression in rats central nervous system following tiletamine anesthesia. Tiletamine action sites in CNS were studied, and general anesthetic mechanisms were also discussed. 72 SD rats were randomly divided into 8 groups. At 0, 10, 30, 60, 90, 100, 120 and 180 min after given tiletamine 50 mg·kg^-1 intraperitoneally, rats were perfused with 4% Paraformaldehyde (0.1 mol·L^-1, PB, pH 7.4). The whole brain was separated into cerebral cortex, hippocampus, thalamus, cerebellum and brain stem. Then these brain tissues were dehydrated 24 h by 20% sucrose (4 ℃). Cryosections were 10 μm and Elivision Immunohistochemical technique for Fosprotein was used to observe and count on the Fos-like immunoreactive neurons. In normal saline group, there was few Fos-like immunoreactive neurons appeared. And Fos-like immunoreactive neurons were observed in cerebral cortex, hippocampus, thalamus, cerebellum and brain stem in experimental groups. Fos-like immunoreactive neurons expression increased at 10 min after tiletamine administrated by intraperitoneal injection, peaked at 60 min and decreased at 90 min. At 180 min after injection, Fos-like immunoreactive neurons recovered to base level, which had no significant difference compared with that before given tiletamine (P＞0.05). The results showed that tiletamine induce expression of c-fos oncogene in the rats′ cerebral cortex, hippocampus, thalamus, cerebellum and brain stem. And the cerebral cortex, hippocampus, thalamus, cerebellum and brain stem at the action sites of tiletamine.

Key words: Tiletamine, c-fos oncogene, central nervous system