关键词: 胎儿成纤维细胞, 血清饥饿, 细胞类型, 克隆胚胎

Abstract: Porcine fetal fibroblast cells, ear fibroblast cells and granulosa cells were used as donor in pig cloning. We compared the effects of cell cycle synchronization treatment of fetal fibroblast cells into G0/G1 by serum starvation or contact inhibition. There were no significant difference in percentage of G0/G1 between two treatments, no significant difference in percentage of G0/G1 by serum starvation for 2d and 4d, as the same when by contact inhibition. Serumstarved fetal fibroblast cells or cycling growing subconfluenced cells， round and smooth vs. rough surface cells,different types of somatic cells and fibroblast cells of different individuals were used as nuclear donor in porcine somatic cloning. The results indicated that serum starvation could not improve the development of cloned embryos. Round and smooth cells led to a high fusion rate. Cell types of donor and different individuals impacted on the development of cloned embryos.

Key words: fetal fibroblast cell, serum starvation, cell type, cloned embryo