畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (1): 14-19.

• 遗传繁育 • 上一篇    下一篇

牦牛心脏脂肪酸结合蛋白基因的分子克隆和进化分析

马志杰; 钟金城;陈智华;字向东; 常怀普;张雪琴;罗晓林;魏雅萍;肖玉萍   

  1. 1西南民族大学生命科学与技术学院，成都 610041；2青海省畜牧兽医科学院畜牧研究所，西宁 810016；3中国农业科学院兰州畜牧与兽药研究所，兰州 730050
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-01-25 发布日期:2007-01-25

Molecular Cloning and Evolution Analysis of the Yak Heart Fatty Acid-binding Protein Gene

  1. 1 College of Life Science & Technology, Southwest University for Nationalities, Chengdu 610041, China; 2 Institute of Animal Science, Qinghai Academy of Animal Science and Veterinary Medicine , Xining 810016, China; 3Lanzhou Institue of Animal and Veterinary Pharmaceutics Science， Chinese Academy of Agricultural Sciences, Lanzhou 730050, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-01-25 Published:2007-01-25

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摘要： 对牦牛心脏脂肪酸结合蛋白（HFABP）基因进行了克隆测序，并与GenBank中9个物种相应基因编码区核苷酸序列进行了比对分析，在此基础上采用邻接法、最大简约法和最小进化法构建了牦牛与其它物种间分子系统进化树。结果表明，牦牛HFABP基因由4个外显子和3个内含子组成，外显子1、外显子2、外显子3 和外显子4大小分别为73、173、102和54 bp，内含子1、内含子2和内含子3大小分别为3 460、1 892和1 495 bp。CDS序列全长为402 bp，前体氨基酸数为133个。不同物种间在该基因核苷酸序列上有较高的保守性。牦牛与普通牛、绵羊、山羊、猪、人、大鼠、小鼠、鸡、斑马鱼各物种在HFABP基因编码区核苷酸序列上同源性大小分别为998%、978%、970%、928%、888%、833%、831%、764%、687%。通过邻接法、最大简约法和最小进化法用HFABP基因编码区核苷酸序列构建的物种间分子系统进化树,结果表明，3种方法构建的物种间分子系统进化树基本一致。系统树总体分为两支，斑马鱼为独立的一支，而牦牛与其它物种为另一大分支。牦牛与普通牛、绵羊与山羊先分别聚在一起，然后再聚为一类；后与猪、人依次聚为一类。小鼠和大鼠先聚为一类，再与人和其它物种聚类，然后再与鸡聚为一类。该系统聚类结果与动物学分类一致，表明HFABP基因适合于构建不同物种间的系统进化树。

关键词: 牦牛, H-FABP基因, 克隆, 分子系统进化

Abstract: The yak heart fatty acidbinding protein (HFABP) gene was cloned and compared the nucleotide sequence of coding region with the homologous HFABP genes in other species including cow, sheep, goat, pig, human, rat, mouse, chick and zebrafish retrieved from the GenBank, and finally a phylogenetic tree was constructed by NJ, MP, ME methods using the coding region DNA sequences of the HFABP genes in all species. The results showed that the yak HFABP gene had 4 exons and 3 introns. The size of exon 1, exon2, exon3 and exon4 was 73,173,102 and 54 bp respectively; while the size of intron 1, intron2 and intron3 was 3 460,1 892 and 1 495 bp respectively.The size of CDS sequence of the yak HFABP gene was 402 bp which encoding 133 amino acids. The nucleotide sequences among different species were quite conservative. The homologies of the coding region of HFABP genes between the yak and cow, sheep, goat, pig, human, rat, mouse, chick and zebrafish were 998%, 978%, 970%, 928%, 888%, 833%, 831%, 764%, 687% respectively. The molecular phylogenetic tree among species was constructed according to the nucleotide sequence of the coding region of HFABP gene. The result indicated that the molecular phylogenetic tree had two branches, branch 1 contained Zebrafish, branch 2 contained yak and other species. Yak and cow, sheep and goat assembled separately, then assembled to a genus, and then assembled to a genus with pig and human, while the rat and mouse assembled to a genus, then assembled to a genus with human, and then chick. This result of phylogenetic clustering was identical to the zoological classification system, indicating that the HFABP gene was also fit to construct molecular phylogenetic tree among different species.

Key words: yak, H-FABP gene, cloning, molecular phylogenetic evaluation

ISSN 0366-6964
CN 11-1985/S
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