畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (7): 1028-1036.

• 动物营养 • 上一篇    下一篇

瘤胃灌注乙丙酸不同摩尔比混合挥发性脂肪酸对奶山羊乳脂合成的影响

程光民,林雪彦,李福昌,陈凤梅,王中华*,刘建胜,伏桂华
  

  1. 山东农业大学动物科技学院,泰安 271018
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-07-24 发布日期:2009-07-24
  • 通讯作者: 王中华

The Influence of Acetate and Propionate Molar Ration in Ruminally Infused Volatile Fatty Acid Mixtures on Milk Fat Synthesis in Lactating Goats

CHENG Guangmin, LIN Xueyan, LI Fuchang, CHEN Fengmei,
WANG Zhonghua﹡,LIU Jiansheng, FU Guihua   

  1. College of Animal Science, Shandong Agricultural University, Taian 271018,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-24 Published:2009-07-24
  • Contact: WANG Zhonghua

摘要: 4只安装永久性瘤胃瘘管和颈动脉插管的泌乳中后期文登奶山羊,采用4×4拉丁方试验设计,试验处理为瘤胃连续灌注4种不同比例的混合挥发性脂肪酸(VFA)溶液,4种灌注液的乙酸/丙酸/丁酸摩尔比依次为:75∶15∶10(VFA1),65∶25∶10(VFA2),55∶35∶10(VFA3)和45∶45∶10(VFA4)。日粮(精粗比为45∶55)制作为颗粒料,每2 h自动饲喂1次,瘤胃灌注的VFA按奶山羊维持需要的30%确定。研究表明,灌注液对奶产量、乳糖的影响不显著(P>005),高丙酸灌注组乳脂率、乳脂量显著下降(P<005);血浆胰岛素水平呈上升趋势(P>005),血糖浓度处理间无显著变化(P>005)。随灌注液丙酸摩尔比的升高,颈动脉血浆乙酸、NEFA、BHBA浓度下降(P<005),丙酸浓度上升(P<005),TG和丁酸浓度组间差异不显著(P>005);动脉血浆BHBA、NEFA、TG和丁酸的乳腺吸收率降低(P<001),丙酸的乳腺吸收率升高(P<001),乙酸的乳腺吸收率未出现显著变化(P>01)。随灌注液丙酸摩尔比的升高,乳脂中中链脂肪酸(C10C16)和中短链脂肪酸(C4C16)的含量呈上升趋势(P<005),长链脂肪酸(≥C16)的含量呈下降趋势(P<005),其中VFA1组C10C16及C4C16含量均低于其它3组(P<005),≥C16含量显著高于其它3组(P<005);Trans10,cis12CLA无显著变化(P>005)。研究结果表明,提高瘤胃灌注VFAs混合液的丙酸摩尔比可引起乳脂率的降低,抑制乳腺对NEFA的吸收,增加乳脂中C4C16脂肪酸的比例,降低≥C16脂肪酸的比例。4只安装永久性瘤胃瘘管和颈动脉插管的泌乳中后期文登奶山羊,采用4×4拉丁方试验设计,试验处理为瘤胃连续灌注4种不同比例的混合挥发性脂肪酸(VFA)溶液,4种灌注液的乙酸/丙酸/丁酸摩尔比依次为:75∶15∶10(VFA1),65∶25∶10(VFA2),55∶35∶10(VFA3)和45∶45∶10(VFA4)。日粮(精粗比为45∶55)制作为颗粒料,每2 h自动饲喂1次,瘤胃灌注的VFA按奶山羊维持需要的30%确定。研究表明,灌注液对奶产量、乳糖的影响不显著(P>005),高丙酸灌注组乳脂率、乳脂量显著下降(P<005);血浆胰岛素水平呈上升趋势(P>005),血糖浓度处理间无显著变化(P>005)。随灌注液丙酸摩尔比的升高,颈动脉血浆乙酸、NEFA、BHBA浓度下降(P<005),丙酸浓度上升(P<005),TG和丁酸浓度组间差异不显著(P>005);动脉血浆BHBA、NEFA、TG和丁酸的乳腺吸收率降低(P<001),丙酸的乳腺吸收率升高(P<001),乙酸的乳腺吸收率未出现显著变化(P>01)。随灌注液丙酸摩尔比的升高,乳脂中中链脂肪酸(C10C16)和中短链脂肪酸(C4C16)的含量呈上升趋势(P<005),长链脂肪酸(≥C16)的含量呈下降趋势(P<005),其中VFA1组C10C16及C4C16含量均低于其它3组(P<005),≥C16含量显著高于其它3组(P<005);Trans10,cis12CLA无显著变化(P>005)。研究结果表明,提高瘤胃灌注VFAs混合液的丙酸摩尔比可引起乳脂率的降低,抑制乳腺对NEFA的吸收,增加乳脂中C4C16脂肪酸的比例,降低≥C16脂肪酸的比例。

关键词: 乳脂, VFAs, 胰岛素, 葡萄糖, 奶山羊

Abstract: Four midlate lactation Wendeng goats equipped with permanent ruminal fistulae and temporary carotid and mammary vein catheters were assigned into a 4×4 Latin square experiment. The four volatile fatty acid mixtures (VFAs) were ruminally infused with different acetate/propionate/butyrate molar ratios (75∶15∶10,VFA1; 65∶25∶10,VFA2; 55∶35∶10,VFA3; 45∶45∶10,VFA4). The food each day was delivered equally in particle by automatic feeder per 2 h and the ratios of concentrate to forage was 45∶55. Among them, the diet should meet the demand of the sheep, the VFAs infused provides of an additional 30% of MEm. The present study results showed that milk yields and milk lactose content were not influenced , but milk fat percent and yields decreased significantly by treatments (P<005). Plasma insulin increased insignificantly and there was no effect on blood glucose(P>005). With the propionate molar ratios increasing in the VFAs, arterial acetate,NEFA and BHBA consistency decreased significantly(P<005), arterial propionate increased(P<005), but TG or butyrate was not changed in treatments (P>005). In the meantime, mammary uptake ratios of BHBA,NEFA,TG and butyrate were decreased(P<001), propionate was increased(P<005), but acetate was no effect (P>005). With increasing propionate molar ratio in the VFAs, contents of short and medium chain fatty acids in milk fat were increased (P<005), and that of long chain fatty acids was decreased significantly (P<005). There was no effect on trans10,cis12 CLA. Results of the present study implied that increased propionate molar ratio in ruminally infused VFAs could decrease milk fat content, depress the mammary uptake of NEFA, increase the contents of short and medium chain fatty acids and decrease long chain fatty acids in milk fat.

Key words: milk fat, VFAs, insulin, glucose, lactating goat