关键词: 37kDa/67kDa LRP/LR, RealTime PCR, mRNA, 绵羊, PrPSc, 复制

Abstract: The aim of the present study was to detect the mRNA transcription level of prion receptor-37kDa/67kDa LRP/LR in ovine tissues. The realtime RTPCR was employed for the quantitation of mRNA transcription level of 11 tissues from six sheep with the same background. The synthesis of cDNA for each sample was firstly performanced by RTPCR after the isolation of RNA from various tissues in sheep. The results showed that the highest LRP/LR mRNA transcription level was found in neocortex (P<005) , which exhibiting as much as tenfold higher than in lung, followed by heart and obex(P<005); intermedia transcription was occured in hippocampus, cerebellum, spleen, thalamus, mesenteric lymph node; the lower transcription was occured in liver and kidney, and the lung expressed extremely lowest but detectable level of LRP/LR mRNA (P<005). These results indicated that altered expression profiles of laminin receptor in various ovine normal tissues are basically corresponding to the degree of PrPSc accumulation, and tissues expressing high levels of LRP/LR may have higher potential susceptibility to PrPSc. Our results also suggest that in ovine tissues， the degree of PrPSc propagation was correlated with the expression level of prion receptor，37kDa/67kDa LRP/LR.

Key words: 37kDa/67kDa LRP/LR, realTime PCR, mRNA, sheep, PrPSc, replication/propagation