关键词: 脂肪细胞, 脂肪生成, 脂解, 转录

Abstract: Rat preadipocytes were isolated by collagenase treatment of epididymal adipose tissue from male SD rats (20-day-old), and differentiated into adipocytes. After induced with experiment model of serumdeprivation/reserum culture, the lipogenesis, lipolysis and relative levels of lipid metabolicrelated gene mRNAs in mature adipocytes were measured through Oil Red O staining, glycerol reagent kit and semiquantitative RTPCR, respectively. The results showed that mRNA levels of hormonesensitive lipase (HSL) were increased according to lasting time of serumdeprivation (P<0.05), and significantly decreased with reserum culture (P<0.05), which coincided with the changes of lipolytic activity in adipocytes. Lipogenesis and mRNA levels of lipogenic genes such as fatty acid synthase (FAS), acetylCoA carboxylase α(ACC1) and carbohydrate response element binding protein (ChREBP) were coordinately reduced according to lasting time of serumdeprivation (P<0.05), and increasingly increased during reserum medium culture (P<0.05). Sterol regulatory element binding protein (SREBP)1c mRNA levels were reduced by serumdeprivation (P<0.05), but did not have significant difference between 36 h and 72 h of serumdeprivation (P>0.05). SREBP1c mRNA levels were not changed by reserum medium culture 36-72 h after 72 h serum-deprivation (P>0.05). It can be concluded that lipogenesis and lipogenic genes mRNA levels coincide with mRNA levels of ChREBP, but do not with that of SREBP-1c, suggesting that ChREBP could be relative to transactivation of FAS and ACC1 in adipocytes, which needs to study further in translation level.

Key words: adipocyte, lipogenesis, lipolysis, transcription