E-钙粘蛋白抗体对小鼠早期胚胎发育的影响

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (8): 1222-1229.doi:

E-钙粘蛋白抗体对小鼠早期胚胎发育的影响

吴兴龙¹，王棉娟²，胡春超¹，王鹏博¹，李相运^1*

1. 河北农业大学动物科技学院，保定 071000； 2. 河北农业大学校医院，保定 071000

收稿日期:2011-08-15 修回日期:1900-01-01 出版日期:2012-08-24 发布日期:2012-08-24
通讯作者: 李相运

Effects of Ecadherin Antibody on Mouse Early Embryo Development

WU Xinglong ¹, WANG Mianjuan², HU Chunchao¹, WANG Pengbo¹, LI Xiangyun^1*

1. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China; 2. Hospital of Agricultural University of Hebei, Baoding 071000, China

Received:2011-08-15 Revised:1900-01-01 Online:2012-08-24 Published:2012-08-24
Contact: LI Xiangyun

摘要/Abstract

摘要： 旨在研究E钙粘蛋白抗体（ECCD1）对小鼠早期胚胎体内外发育的影响。在体外培养小鼠8细胞胚胎的培养液中，分别添加不同浓度的ECCD1，通过胚胎形态观察、碱性磷酸酶染色、多能性因子免疫染色、体外贴壁培养、嵌合体制备以及体内移植试验检测ECCD1对小鼠胚胎发育能力的影响。结果表明，ECCD1抗体延迟胚胎致密化和囊胚腔形成，但内细胞团并未受到影响，胚胎碱性磷酸酶和SSEA1、Oct4、Sox2、Nanog 4种多能性因子的免疫染色都呈阳性，此外，ECCD1抗体也不影响胚胎的体外贴壁生长、体内移植着床以及嵌合体的制备。这些数据表明，ECCD1抗体延迟胚胎致密化但并不影响胚胎的体内外发育能力。

Abstract: To investigate the effects of Ecadherin antibody (ECCD1) on mouse embryo development, ECCD1 antibody with different concentrations was added into KSOM media when mouse 8cell embryos were cultured in vitro, and then the developmental potential of ECCD1treated embryos were detected by morphology, alkaline phosphatase activity, pluripotent marker (SSEA1, Oct4, Sox2, Nanog) immunostaining, attachment culture in vitro, implantation in vivo, and chimera production. The result showed that ECCD1 significantly delayed embryo compaction and blastocoele formation. ECCD1treated embryos had obvious inner cell mass, positive alkaline phosphatase and pluripotent markers, normal attachment growth in vitro and implantation in vivo. In addition, the chimera production was also improved when 8cell embryos injected by embryonic stem cells were treated with ECCD1. The results indicated that compaction delay by ECCD1 antibody did not affect embryo development potential.

吴兴龙;王棉娟;胡春超;王鹏博;李相运. E-钙粘蛋白抗体对小鼠早期胚胎发育的影响[J]. 畜牧兽医学报, 2012, 43(8): 1222-1229.

WU Xinglong;WANG Mianjuan;HU Chunchao;WANG Pengbo;LI Xiangyun . Effects of Ecadherin Antibody on Mouse Early Embryo Development[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(8): 1222-1229.

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