Abstract:

The aim of this study was to detect the expression of miR155 in skeletal muscle development in Landrace (leantype) and Tongcheng (obesetype) pigs, and explore the roles of miR155 in muscle development. Based on the skeletal muscle tissues of 22 stages (12 prenatal and 10 postnatal stages) in Landrace and Tongcheng pigs, respectively, stemloop RTPCR was performed to detect the miR155 expression. Furthermore, the expression of miR155 in various tissues of adult Tongcheng pigs was detected. The miR155 differential expression in skeletal muscle development exhibited different expression patterns between Landrace and Tongcheng pigs. In adult Tongcheng pigs, miR155 was abundantly expressed in small intestines and lung, moderately in heart and placenta, and weakly in other collected tissues. The result indicate that miR155 may be involved in myofibre proliferation and differentiation and further contribute to skeletal muscle development and muscle phenotype with different genotypes.