Abstract:

A study on thiamphenicol （TAP） residue depletion was conducted in chicken muscle. TAP was extracted from chicken muscle with acetone and dichloromethane. The extract solution was degreased in nhexane saturated with acetonitrile, dried in nitrogen evaporator and residues were dissolved in mobile phase. TAP was determinated by high performance liquid chromatography (HPLC) with fluorescence detector. The limits of detection （LODs） was 1.5 μg·kg-1 (S/N=3) and the limits of quantitation（LOQs）was 5 μg·kg-1 (S/N=10) for TAP in chicken muscle, respectively. After the chickens orally administered successively TAP capsules of 10.0, 20.0 and 50.0 mg·kg-1 of body weight once every day for 5 days, respectively, residues of TAP in chicken muscle had a rise trend. The maximum residue of TAP was detected at the first day after withdrawal time in chicken muscle. At the early days after withdrawal time, the residues of TAP in chicken muscle were eliminated faster, but they were eliminated slowly at the later period. Residues of TAP in chicken muscle were all lower than 50 μg·kg-1 at the 5th withdrawal day, and all lower than the lowest LODs (1.5 μg·kg-1）at the 9th withdrawal day. Residues of TAP in chicken muscle were positively correlated with TAP orally administered doses.