Abstract:

The objective of this study was to get effective strain of nematodetrapping fungusArthrobotrys oligospora, by which parasitic nematodes of livestocks could be controlled and chemical drugs may be replaced partly or completely. First of all，the mutagenic effect of the lowenergy nitrogen ion beam on the original strain  A. oligospora were studied, then the survival rate of strains injected by different doses of nitrogen ion were determined to select the best injection parameter: energy, dose. Later, the strains were screened out according to mycelial growth rate, sporulation, the trapping rate and resistance to the digestion of the sheep. The results showed that ion beam implantation could be an efficient way to improve the characters of nematodetrapping fungus, such as growth rate，sporulation quantity and nematodetrapping rate, and it was the first time to use ion beam in mutation breeding of nematodetrapping fungi.