Abstract:

Spleen is the target organ of Newcastle disease virus (NDV). To elucidate the interaction between NDV and its hosts, and to further evaluate the pathological differences of spleen from the geese infected by the earlygenotype NDV strain Herts/33 (IV) and recentgenotype NDV strain JS5/05 (VIId), the comparative proteomes of spleen from geese infected with these two NDVs were analyzed using twodimensional gel electrophoresis (2DE). Thirtysix 30dayold geese were divided into three groups. The spleens from the geese in different groups were collected at 36 hours, 72 hours and 108 hours post infection. The soluble proteins were extracted and then separated with 17 cm IPG strips (pH 58). Moreover, the 2DE maps were comparatively analyzed by the software PDQuest 8.0.1. Compared with the control group, 154 and 148 differentially expressed protein spots were found in Herts/33 and JS5/05infected group, respectively. There were 86 spots shared by the two NDVinfected groups, with 52 proteins upregulated and 34 proteins downregulated. In addition, a total of 130 differentially expressed protein spots were identified between Herts/33 and JS5/05infected groups, including 71 upregulated proteins and 59 downregulated proteins. The virulent genotype Ⅳ and genotype Ⅶd NDVs induced distinct splenic protein expression profiles after infection, which provided useful information to further elucidate the molecular mechanisms of increased pathogenicity of genotype Ⅶd NDV to waterfowl.