1株猪繁殖与呼吸综合征病毒ORF5基因缺失毒株的基因组特征与演化分析

畜牧兽医学报

1株猪繁殖与呼吸综合征病毒ORF5基因缺失毒株的基因组特征与演化分析

付向晶，王丹阳，王兴龙，张渭东，宋祥军，刘阳坤，唐文雅，慕国辉，刘海金，贺生芳，杜恩岐，杨增岐^*

(西北农林科技大学动物医学院, 杨凌 712100)

收稿日期:2012-04-17 出版日期:2012-11-26 发布日期:2012-11-26
通讯作者: 杨增岐（1963-），男，陕西岐山人，教授，博导，主要从事动物传染病学研究，E-mail: yzq8162@163.com
作者简介:付向晶（1985-），女，河南开封人，博士，主要从事动物传染病学研究，E-mail: jingjing_kuaile@163.com;王丹阳（1987-），男，山东临朐人，硕士，主要从事动物传染病学研究，E-mail: xibeiadan@163.com。二人并列第一作者
基金资助:
“十一五”国家科技支撑计划项目（2006BAD06A08）

Genomic Characterization and Evolvement Analysis of a Porcine Reproductive and Respiratory Syndrome Virus Isolate with Deletion in GP5

FU Xiang-jing, WANG Dan-yang, WANG Xing-long, ZHANG Wei-dong, SONG Xiang-jun, LIU Yang-kun, TANG Wen-ya, MU Guo-hui, LIU Hai-jin, HE Sheng-fang, DU En-qi, YANG Zeng-qi^*

(Northwest A & F University, College of Veterinary Medicine, Yangling 712100, China)

Received:2012-04-17 Online:2012-11-26 Published:2012-11-26

摘要/Abstract

摘要： 为了解陕西猪场猪繁殖与呼吸综合征病毒（PRRSV）的变异情况，对发病猪场进行PRRSV分离，采用RT-PCR方法对分离株NSP2基因和ORF5基因分别进行扩增，测序后进行序列分析。结果显示：得到1株PRRSV，命名为HZ1007株，属于美洲型；其NSP2 481位和533—561位存在30个氨基酸的不连续缺失，与高致病性PRRSV分子特征相似；GP5 85—95位氨基酸处出现了11个氨基酸的连续缺失；系统进化分析发现HZ1007与国内高致病性代表毒株HUN4、JXwn06、SY0608等亲缘关系较近，且与HUN4株相似性最高，为97.4%。本研究发现了GP5缺失型PRRSV，这在国内外尚属首例，且HZ1007株与高致病性PRRSV亲缘关系较近，在系统进化分析树上与高致病性毒株同属一个分支，其可能是高致病毒株新的变异型，其致病性和生物学特性需进一步研究分析。

Abstract: In order to understand the variation of porcine reproductive and respiratory syndrome virus (PRRSV) in Shaanxi province, the virus was detected from swine clinical samples, NSP2 and ORF5 genes were amplified, cloned and sequenced for analyzing the genetic variation of PRRSV. The result showed that the strain belonged to the North American genotype, named HZ1007. It had discontinuous 30 amino acids deletion in the aa position of 481 and 533-561 of NSP2, which was the same deletions with the highly pathogenic PRRSV. Moreover, the ORF5 gene of HZ1007 strain contained a continuous 11 amino acids deletion in the aa positon of 85-95. PRRSV HZ1007 was highly homologous to HUN4 and SY0608 strains. Phylogenetic tree analysis revealed that HZ1007 had closer relationship with the highly pathogenic PRRSV than classical strains, it may be a new variant of the highly pathogenic strain. The athogenic force and biological characteristics needed further research and analysis.

中图分类号:

S852.659.6

付向晶，王丹阳，王兴龙，张渭东，宋祥军，刘阳坤，唐文雅，慕国辉，刘海金，贺生芳，杜恩岐，杨增岐. 1株猪繁殖与呼吸综合征病毒ORF5基因缺失毒株的基因组特征与演化分析[J]. 畜牧兽医学报, doi: .

FU Xiang-jing, WANG Dan-yang, WANG Xing-long, ZHANG Wei-dong, SONG Xiang-jun, LIU Yang-kun, TANG Wen-ya, MU Guo-hui, LIU Hai-jin, HE Sheng-fang, DU En-qi, YANG Zeng-qi. Genomic Characterization and Evolvement Analysis of a Porcine Reproductive and Respiratory Syndrome Virus Isolate with Deletion in GP5[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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参考文献

［1］ZHOU L, YANG H. Porcine reproductive and respiratory syndrome in China［J］.Virus Res, 2010, 154(1-2):31-37.

［2］郭宝清，陈章水，刘文兴，等. 从疑似 PRRS 流产胎儿分离 PRRSV 的研究［J］. 中国畜禽传染病，1996, 18(2): 1-4.

［3］DEA S, GAGNON C A,MARDASSI H. Antigenic variability among North American and European strain of porcine reproductive and respiratory syndrome virus as defined by monoclonal antibodies to the matrix protein［J］.Clin Microbiol,1996,34(6):1488-1493.

［4］MURTAUGH M P, ELAM M R, KAKACH L T. Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus［J］.Arch Virol,1995,140:1451-1460.

［5］FORSBERG R, STORGAARD T, NIELSEN H S, et al. The genetic diversity of European type PRRSV is similar to that of the North American type but is geographically skewed with in Europe［J］.Virology,2002,299:38-47.

［6］STADEJEK T,STANKEVICIUS A,STORGAARD T, et al. Identification of radically different variants of porcine 19 reproductive and respiratory syndrome virus in Eastern Europe: towards a 20 common ancestor for European and American viruses ［J］.J Gen Virol, 2002,83:1861-1873.

［7］NELSEN C J,MURTAUGH M P,FAABERG K S. Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents［J］.J Virol,1999,73(1):270-280

［8］MEULENBERG J J, MEUER E J, MOORMANN R J. Subgenomic RNAs of Lelystad virts contain a conserved leaderbody junction sequence［J］.J Gen Virol,1993,74(8):1697-1701.

［9］STADEJEK T, OLEKSIEWICZ M B, POTAPCHUK D, et al. Porcine reproductive and respiratory syndrome virus strains of exceptional diversity in Eastern Europe support the definition of new genetic subtypes［J］.J Gen Virol, 2006,87:1835-1841.

［10］MATEU E,DIAZ I,DARWICH L, et al. Evolution of ORF5 of Spanish porcine reproductive and respiratory syndrome virus strains from 1991 to 2005 ［J］.Virus Res,2006,115(2):198-206.

［11］MATEU E, MARTIN M, VIDAL D. Genetic diversity and phylogenetic analysis of glycoprotein 5 of Europeantype porcine reproductive and respiratory virus strains in Spain ［J］.J Gen Virol,2003,84:529-534.

［12］ZHOU L, ZHANG J L, ZENG J W, et al. The 30-amino-acid deletion in the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence［J］. J Virol, 2009, 83(10): 5156-5167.

［13］GAO Z Q, GUO X, YANG H C. Genomic characterization of two Chinese isolates of porcine respiratory and reproductive syndrome virus［J］. Arch Virol, 2004, 149:1341-1351.

［14］ALLENDE R, LEWIS T L, LU Z, et al. North American and European porcine reproductive and respiratory syndrome viruses differ in non-strictural protein coding regions ［J］. J Gen Virol,1999,80(2):307-315.

［15］OSTROWSKI M, GALEOTA J A, JAR A M, et al. Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain ［J］.J Virol, 2002, 76:4241-4250.

［16］童光志，周艳君，郝晓芳.高致病性猪繁殖与呼吸综合征病毒的分离鉴定及其分子流行病学分析［J］.中国预防兽医学报，2007, 29（5）：323-326.

［17］ANSARI I H, KWON B, OSORIO F A, et al. Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity,antigenicity,and ability to induce neutralizing antibodies ［J］.J Vitol, 2006,80:3994-4004.

［18］TJEERD G K, LISETTE A C,ROB J M, et al. Challenges for porcine reproductive and respiratory syndrome virus(PRRSV) vaccinology ［J］.Vaccine, 2009, 27(28):3704-3718.

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