鸡前脂肪细胞中A-FABP基因表达的变化对PPARγ、perilipin和E-FABP表达的影响

畜牧兽医学报

鸡前脂肪细胞中A-FABP基因表达的变化对PPARγ、perilipin和E-FABP表达的影响

张庆秋^1,2，石慧^1,2，王宇祥^1,2，李辉^1,2*，王启贵^1,2*

（1.农业部鸡遗传育种重点实验室，哈尔滨 150030； 2. 东北农业大学动物科学技术学院，哈尔滨 150030）

收稿日期:2012-02-01 出版日期:2012-10-25 发布日期:2012-10-25
通讯作者: 李辉，E-mail: lihui@neau.edu.cn；王启贵，E-mail: wangqigui@hotmail.com
作者简介:张庆秋（1984-），女，辽宁岫岩人，硕士，主要从事家禽遗传育种研究，E-mail: qiuzhilian.1984@163.com
基金资助:
国家自然科学基金项目（30771542；30972087）；现代农业产业技术体系建设项目(CARS-42)黑龙江省高等学校科技创新团队建设项目（2010td02）

The Effect of A-FABP Gene Overexpression or Knockdown on the Expression of PPARγ, perilipin and E-FABP in Chicken Adipocytes

ZHANG Qing-qiu^1,2, SHI Hui^1,2, WANG Yu-xiang^1,2, LI Hui^1,2*, WANG Qi-gui^1,2*

（1. Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture，Harbin 150030,
China; 2. College of Animal Science and Technology, Northeast Agricultural University,
Harbin 150030, China）

Received:2012-02-01 Online:2012-10-25 Published:2012-10-25

摘要/Abstract

摘要： 本研究以鸡原代前脂肪细胞为实验材料，探讨A-FABP基因在鸡脂类代谢过程中的功能以及该基因与脂类代谢相关7个基因间的调控关系。利用A-FABP基因的shRNA干扰载体和真核表达载体对该基因进行干扰和过表达，分别在干扰和过表达后24、36、48、60和72 h时检测脂类代谢相关基因的表达情况。结果表明，干扰A-FABP后，鸡前脂肪细胞中PPARγ和perilipin基因的表达量在24和36 h时显著低于对照组，在48 h时显著高于对照组，在48和72 h时，E-FABP基因表达量显著低于对照组；过表达A-FABP后，鸡前脂肪细胞中PPARγ和perilipin基因的表达量在24和36 h时显著高于对照组，在48 h时显著低于对照组，在所有检测的时间点E-FABP基因表达均显著下调。结果显示，在前脂肪细胞中，鸡A-FABP表达量的变化影响PPARγ、perilipin和E-FABP的表达。

Abstract: This study was designed to investigate the role of chicken A-FABP gene in lipid metabolism of adipocytes and the regulation relationship between A-FABP gene and the genes related to lipid metabolism. The shRNA interference vector with A-FABP gene and eukaryotic expression vector were used. Chicken adipocytes with short hairpin RNA-mediated knockdown and A-FABP cDNA overexpression were used. The genes expression level in chicken adipocytes were detected at 24, 36, 48, 60 and 72 h after transfection with the interference and overexpression plasmids of A-FABP. The results showed that the expression levels of PPARγ and perilipin genes were downregulated at 24 and 36 h and upregulated at 48 h in the A-FABP interference group. The expression levels of E-FABP genes was downregulated at 48 and 72 h in the A-FABP interference group. The expression levels of PPARγ and perilipin genes were upregulated at 24 and 36 h and downregulated at 48 h in the A-FABP overexpression group. The expression levels of E-FABP genes was downregulated at all detected time pionts in the A-FABP overexpression group. These results indicated that A-FABP might affect expression of PPARγ, perilipin and E-FABP in chicken adipocytes.

中图分类号:

S831.2

张庆秋，石慧，王宇祥，李辉，王启贵. 鸡前脂肪细胞中A-FABP基因表达的变化对PPARγ、perilipin和E-FABP表达的影响[J]. 畜牧兽医学报, doi: .

ZHANG Qing-qiu, SHI Hui, WANG Yu-xiang, LI Hui,WANG Qi-gui. The Effect of A-FABP Gene Overexpression or Knockdown on the Expression of PPARγ, perilipin and E-FABP in Chicken Adipocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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