畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (9): 1337-1340.doi:

• 研究简报 • 上一篇    下一篇

西南矮马矮小性状基因SHOX的克隆测序和多态性研究

乌尼尔夫1,塔娜1,孙玉江2,芒来1*   

  1. 1.内蒙古农业大学动物科学学院,呼和浩特 010018; 2. 山东省东营市农业科学研究院,东营 257091
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-09-23 发布日期:2011-09-23
  • 通讯作者: 芒来

Cloning and Study on Polymorphism of Southwest Horse SHOX Gene

Unerhu1, TA Na1,SUN Yu-jiang2, MANG lai1*   

  1. 1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China; 2. Academy of Agricultural Science Institute of Dongying City in Shandong Province, Dongying 257091, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-09-23 Published:2011-09-23
  • Contact: MANG lai1

摘要: 本研究首次克隆出西南马矮小性状基因完整第二外显子序列,GenBank登录号为EU556739;通过PCRSSCP方法对8个品种150匹马的该序列做了多态性分析,在P2位点上268 处有1个 G→A 的突变,和431 处有1个 T→G 的突变导致了等位基因 B 变为等位基因 A。该位点除云南文山马(WS)的优势基因型和优势等位基因分别为AB型和B外,其余类群马均为BB型和B。云南丘北马(QB)、云南文山马(WS)、云南大理马(DL)、建昌马(JC)和设特兰马(ST)在P2位点上处于HardyWeinberg平衡状态。

Abstract: The exon2 sequence of SHOX gene of Southwest horse was cloned in this study, GenBank accession number was EU556739. The analysis of PCRSSCP showed that P2 site existed variations. The transversion of G→A in 268 and T→G at 431 led to the transversion of allele B→A. The dominant allele and genotype of WS were AB type and B, and the dominant allele and genotype of other horse types were BB type and B. P2 site was in HardyWeinberg equilibrium in QB horse, WS horse, JC horse and ST horse.