蜂王浆对初情期前雌性日本大耳兔生长发育的影响

畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (4): 503-507.doi:

蜂王浆对初情期前雌性日本大耳兔生长发育的影响

许宝华^{1, 2}，曾志将¹*

1. 江西农业大学动物科学技术学院, 南昌 330045; 2. 南昌大学实验动物科学部, 南昌 330031

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-04-20 发布日期:2011-04-20
通讯作者: 曾志将

Effect of Royal Jelly on Growth and Development before PubertyFemale Japanese White Rabbits

XU Bao-hua^{1, 2}, ZENG Zhi-jiang¹*

1. College of Animal Science and Technology, Jiangxi Agricultural University,Nanchang 330045, China; 2. Department of Laboratory Animal Science,Nanchang University, Nanchang 330031, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

摘要/Abstract

摘要： 本研究旨在探讨蜂王浆对雌性日本大耳兔初情期前的生长发育影响。将36只50日龄雌性日本大耳兔随机分为高剂量蜂王浆组(H-RJ)、低剂量蜂王浆组(L-RJ)以及对照组(C)，每组12只。高、低剂量组分别按体质量0.2%和0.1%的量每天进行空腹灌胃蜂王浆，对照组灌胃体质量0.1%的生理盐水。每周同一时间空腹称重。40 d后，所有兔子耳静脉取血后各组处死6只，摘取心脏、左侧卵巢和左侧子宫称重，并冷冻保存右侧卵巢和右侧子宫。采用放免法测定血清中雌二醇（E2）、促卵泡素（FSH）、促黄体素（LH）、孕激素（P4）、催乳素（PRL）和睾酮（T）的含量；用实时荧光定量PCR检测右侧卵巢中雌激素β受体（ERβ）mRNA和右侧子宫中雌激素α受体（ERα）mRNA的表达。结果表明：灌胃蜂王浆12 d后，对照组兔子没有换毛现象，低剂量和高剂量组分别有75%和67%的兔子换毛；蜂王浆对体质量和心脏、左侧子宫、左侧卵巢的脏器系数无显著影响（P>0.05）。试验组血液中E2浓度极显著高于对照组（P<0.01)，而其它性激素无明显差异（P>0.05）。试验组ERα在子宫和ERβ在卵巢的表达量均低于对照组，对照组与高剂量组的差异极显著（P<0.01)，但低剂量组与对照组和高剂量组之间均差异不显著（P>0.05）。结果提示，蜂王浆能提高兔子的换毛率，但对心脏、子宫和卵巢的生长发育无明显影响；蜂王浆可以提高初情期前雌性日本大耳兔血清中雌激素水平，但抑制子宫和卵巢雌激素受体的表达。

Abstract: This experiment was conducted to explore the effect of royal jelly(RJ) on growth and development of Japanese White rabbits before puberty. Thirty six 50-day-old female rabbits were randomly allocated to three groups of 12 rabbits each: high dose group（H-RJ）, low dose group(L-RJ) and control group(C). Rabbits were administrated daily with RJ weighted 0.2% and 01% of body weight in the high dose group and low dose group via gastric, respectively; saline water weighted 0.1% of body weight was administrated in the control group via gastric. After 40 days, plasma sample was collected from each rabbit and the levels of E2, FSH, LH, P4, PRL and T were analyzed by radioimmunoassay. The heart, left uterus and left ovary were collected from six slaughted rabbits in each group and the expression of the estrogen receptor alpha (ERα) in right ovary and estrogen receptor beta (ERβ) in right uterus were detected by real time RT-PCR. The results showed that: (1) The molting rate of rabbits in C, L-RJ and H-RJ was 0, 75% and 67% after 12 days, respectively; (2) There was no influence of RJ on the body weight, heart, uterus and ovary coefficients of rabbit （P>0.05); (3) The concentration of E2 was significantly higher in RJ treated groups than that in control group （P<0.01), but no significant difference was observed in the other sex hormones （P>0.05）; (4) The expression of ERα in the uterus and ERβ in the ovary were lower in RJ treated groups than that in control. The results indicated that royal jelly can greatly improve the molting rate of the rabbits, but it did not influence the growth of the heart, uterus and ovary, it increased the serum estrogen level and inhibited the expression of estrogen receptor in uterus and ovary of Japanese White rabbits before puberty.

许宝华;;曾志将. 蜂王浆对初情期前雌性日本大耳兔生长发育的影响[J]. 畜牧兽医学报, 2011, 42(4): 503-507.

XU Bao-hua;;ZENG Zhi-jiang . Effect of Royal Jelly on Growth and Development before PubertyFemale Japanese White Rabbits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(4): 503-507.

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