应用微卫星DNA标记分析荷斯坦母牛系谱可靠性及影响因素

畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (2): 163-168.doi:

应用微卫星DNA标记分析荷斯坦母牛系谱可靠性及影响因素

初芹^1,2，张毅¹，孙东晓¹，俞英¹，王雅春¹，张沅¹*

1. 中国农业大学动物科技学院，北京 100193；2. 北京市农林科学院畜牧兽医研究所，北京 100097

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-02-25 发布日期:2011-02-25
通讯作者: 张沅

Pedigree Verification of Holstein Cows and Analysis of Factors Affecting Incorrect Paternity Based on Microsatellite Markers

CHU Qin^1,2, ZHANG Yi¹, SUN Dong-xiao¹, YU Ying¹, WANG Ya-chun¹, ZHANG Yuan¹*

1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-02-25 Published:2011-02-25

摘要/Abstract

摘要： 为了评估当前中国荷斯坦牛群体的系谱准确性，本研究选择15头种公牛及来自21个牛场的2 220头女儿牛，借助牛3号染色体（BTA3）上17个微卫星标记，对女儿-公牛标记基因型是否错配进行分析。结果，按照错配标记数大于等于3个为系谱错误的标准，本研究所涉及的母牛群中系谱错误率为16.62%。经对影响系谱错误的因素分析表明，场效应是主要的影响因素，不同场间系谱错误比例差异较大；由于公牛女儿在各牛场中分布不均匀，导致公牛效应也达到显著。研究结果提示在中国荷斯坦牛群体进行亲子鉴定是亟需和必要的。

Abstract: To verify the pedigree data of Chinese Holstein cows,17 microsatellites on BTA3 were genotyped in 15 Chinese Holstein sires and their 2 220 daughters, which came from 21 herds in Beijing. The paternity with conflicts between the putative sire and daughter in at least 3 markers was considered to be erroneous. The paternity error rate was 16.62% in the Chinese Holstein population. Factors affecting incorrect paternity were further analyzed and results showed that the paternity error rate varied extremely significantly among herds. Since the daughters of sires were uneven distributed in different farms, the effect of sire was also significant. These findings indicate that it’s urgent to carry out paternity testing in the Chinese Holstein population.

初芹;张毅;孙东晓;俞英;王雅春;张沅. 应用微卫星DNA标记分析荷斯坦母牛系谱可靠性及影响因素[J]. 畜牧兽医学报, 2011, 42(2): 163-168.

CHU Qin;ZHANG Yi;SUN Dong-xiao;YU Ying;WANG Ya-chun;ZHANG Yuan . Pedigree Verification of Holstein Cows and Analysis of Factors Affecting Incorrect Paternity Based on Microsatellite Markers [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(2): 163-168.

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