Abstract: Four monoclonal antibodies against Goose Parvovirus (GPV) VP3 already obtained by our laboratory were used for the exact location to linear B-cell epitopes against VP3 of GPV. On the basis of the epitopes results of GPV VP3 protein that have already been identified in previous study, screened epitopes which can be identified by monoclonal antibodies directly. Oligomeric nucleic acid fragments of ten amino acid fragments mutually coinciding five amino acids were contrived, after annealing, connected with pET-32a, by allaxis identification, derivation expressed, small fragment fusion proteins were obtained. The antigenicity was identified by Western blot with McAbs. With the same method, amino acids were deleted one by one, and two epitopes were located at 430-435 aa and 643-647 aa.