Abstract: The experiment was conducted to investigate the effects of bee pollen (BP) and its polysaccharides (PS) on growth performance, nutrient digestibility and serum biochemical indexes in calves. 25 newborn Holstein calves were randomly allocated to 5 treatments with 5 calves each. A commercial milk replacer were fed to the calves. The five treatments were 0 g·d^-1(C), 10 g·d^-1 (10BP), 25 g·d^-1 (25BP), 50 g·d^-1 (50BP) of bee pollen and 5 g·d^-1 (5PS) of bee pollen polysaccharides substituted for milk replacer in equal amounts. The experiment started on 14 d after born and lasted for 56 days, using anus fecal method to determine the apparent digestibility of nutrient in two periods (21-28 d, 42-49 d).The results showed that. average daily gain of calves were significantly higher in 25BP and 5PS groups than that in C group（P<0.05). F/G decreased by 12.85% in 25BP group compared with that in C group（P<0.05);Apparent digestibility rate of dry matter in 21-28 d increased by 8.38% and 7.66% in 25BP and 5PS groups respectively compared with that in C group（P<0.05), meanwhile the apparent digestibility of crude protein increased by 18.03% in 25BP group（P<0.05); Serum TP and ALB content of calves fed bee pollen or polysaccharides were slightly increased, and BUN and CHO content were decreased. TG content were significantly decreased in treatment groups compared with that in C group(P<005). Supplement of bee pollen and polysaccharides in calves diet could improve the growth performance of calves, apparent digestibility rate of dry matter and crude protein, slightly increased serum TP and ALB content, reduced feed conversion ratio and serum triglyceride level significantly. Bee pollen additive in 25 g·d^-1 and polysaccharides in 5 g·d^-1 in milk replacer could get better performance and higher apparent digestibility of calves.