山羊GDF9基因在卵丘卵母细胞复合体体外成熟过程中的表达

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (6): 671-677.doi:

山羊GDF9基因在卵丘卵母细胞复合体体外成熟过程中的表达

郑爱燕^1,2，吴曼¹，尚利青¹，丁家桐¹*

1. 扬州大学动物科学与技术学院，扬州 225009；2. 苏州市立医院生殖与遗传中心，苏州 225000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-06-25 发布日期:2010-06-25
通讯作者: 丁家桐

Expression of Growth Differentiation Factor 9 in Goat COCs during in vitro Maturation

ZHENG Ai-yan^1,2, WU Man¹, SHANG Li-qing¹, DING Jia-tong¹*

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2.The Center for Reproduction and Heredity, Suzhou Medicine Hospital, Suzhou 225000,China

Received:1900-01-01 Revised:1900-01-01 Online:2010-06-25 Published:2010-06-25

摘要/Abstract

摘要： 本研究采用实时荧光定量PCR技术，从mRNA水平探讨了山羊卵丘卵母细胞复合体体外成熟（IVM）过程中（0、6、12、18、24和27 h）GDF9基因的相对表达变化，分析其与卵丘细胞扩散之间的关系。结果表明，GDF9 mRNA在山羊卵丘卵母细胞复合体体外成熟过程中均有表达，且在成熟培养初期表达量较低，其表达峰值出现在IVM 12 h，与卵丘细胞开始扩散的时间一致，之后又有所下降。由此推测，GDF9基因在山羊卵丘扩散和卵母细胞体外成熟过程中起着重要的作用。

Abstract: To analyze the relationship between GDF9 gene and expansion of cumulusoocyte complex (COCs), the expression of GDF9 in goat COCs during oocytes in vitro maturation (IVM) (0, 6, 12, 18, 24 and 27 h) was detected by fluorescent quantitative RTPCR method. The result showed that GDF9 mRNA were expressed at low levels in immature oocytes and increased to the highest level at 12 h of IVM, which coincides with the time of cumulus cell expansion, then declined slowly during IVM, with a slight increase at the end of IVM. The expression pattern of GDF9 suggested that it might be important in cumulus expansion and oocyte maturation of goat.

郑爱燕;吴曼;尚利青;丁家桐. 山羊GDF9基因在卵丘卵母细胞复合体体外成熟过程中的表达[J]. 畜牧兽医学报, 2010, 41(6): 671-677.

ZHENG Ai-yan;WU Man;SHANG Li-qing;DING Jia-tong. Expression of Growth Differentiation Factor 9 in Goat COCs during in vitro Maturation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(6): 671-677.

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