3种奶牛布鲁氏菌病血清学检测方法的贝叶斯评估

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (4): 449-453.doi:

3种奶牛布鲁氏菌病血清学检测方法的贝叶斯评估

刘晓民，杨华，高明春，李彦伟，王君伟*

东北农业大学动物医学学院，哈尔滨 150030

收稿日期:2009-10-22 修回日期:1900-01-01 出版日期:2010-04-25 发布日期:2010-04-25
通讯作者: 王君伟

Evaluation of Three Serological Tests for Brucellosis in Cattle Using Bayesian Analysis"

LIU Xiaomin, YANG Hua, GAO Mingchun, LI Yanwei, WANG Junwei*

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

Received:2009-10-22 Revised:1900-01-01 Online:2010-04-25 Published:2010-04-25
Contact: WANG Junwei

摘要/Abstract

摘要： 在无“金标准”的情况下，为评估虎红平板凝集试验（RBT）、荧光偏振分析法（FPA）和间接酶联免疫吸附试验（IELISA）用于奶牛布鲁氏菌病的抗体检测能力，作者分别以RBT、FPA、IELISA 3种方法对采自某奶牛场的血清样品进行检测，通过贝叶斯分析法对检测结果进行分析，研究3种方法检测奶牛布鲁氏菌血清抗体的敏感性（Se）和特异性（Sp）。结果显示，RBT、FPA、IELISA 3种检测方法的敏感性（Se）分别为91.81%、95.92%和95.98%；特异性（Sp）分别为98.80%、86.54%和98.65%。本研究表明，3种血清学检测方法中以IELISA的检测能力最好。

Abstract: The objective of our study was to evaluate the diagnostic performance of three serological tests for brucellosis. Serological test results were used to compare the performance of Rose Bengal Test (RBT), indirect ELISA (IELISA) and Fluorescence Polarisation Assay (FPA) in diagnosing brucellosis in cattle. Their performance was evaluated using Bayesian analysis which allows the sensitivity (Se) and specificity (Sp) to be estimated in the absence of a gold standard. The sensitivity of RBT, FPA and IELISA were 91.81%, 95.92% and 95.98%, respectively; and specificity of RBT, FPA and IELISA were 98.80%, 86.54% and 98.65%, respectively. The results demonstrated that the IELISA performing well in diagnosing brucellosis in cattle.

刘晓民;杨华;高明春;李彦伟;王君伟. 3种奶牛布鲁氏菌病血清学检测方法的贝叶斯评估[J]. 畜牧兽医学报, 2010, 41(4): 449-453.

LIU Xiaomin;YANG Hua;GAO Mingchun;LI Yanwei;WANG Junwei. Evaluation of Three Serological Tests for Brucellosis in Cattle Using Bayesian Analysis" [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(4): 449-453.

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