Abstract: In order to find out genes related to special characters of the BlackBone Silky Fowl, differential display PCR was used for screening differential expressed genes between liver organs of the Silkies and the AA broilers, and bioinformatics analysis were carried out to identify ESTs differential expressed. There were totally 10 ESTs (Acc_N: CN606328CN606329, CN606331CN606338) screened from the Silkies′and the AA broilers′liver organs. Results of bioinformatics analysis showed, among 10 ESTs, CN606332 and CN606336 were part of the chicken 18S rRNA gene; CN606333 was homologous with the chicken heat shock 70 ku protein 5 (HSPA5) gene; CN606328 and CN606331 were significant similar with some of chicken cDNA clones or ESTs but had unknown function; the others had no significant similar with any chicken nucleotide sequences but had partly similarity to other species′genes, including genes of membrane protein, gammaglutamyl transpeptidase, ATPbinding cassette transporter, putative protein MGC27019, and abnormal spindle microcephaly protein (ASPM). It was suggested in this study that many genes on varieties of metabolism pathways were differentially expressed between the Silkies and broilers. To study these genes more deeply could provide important information for studying the formation of special characters of the Silkies.