Abstract: The objective of this study was to clone goat Dkk1 gene, identify its polymorphisms and find DNA markers associated with cashmere production To clone goat Dkk1 gene, PCR with primers designed within conserved encoding region was performed. Polymorphisms were screened by PCR amplification and sequencing with two genomic DNA pools of goats differing in cashmere production, and detected by PCR-SSCP approach. A fragment of 3 491 bp in size for goat Dkk1 gene was amplified and sequenced, including four exons and three introns and encoding 265 amino acid residues. It has high homology with the corresponding sequences of other mammalian species both at mRNA and amino acid levels. Altogether 33 single nucleotide substitutions and an insertion of 4 bp in intron 2 were revealed. Two polymorphic sites in (T593C and C603A) the promoter region were detected in five goat populations. Allele A was predominant in all of the five goat populations, and all populations were in Hardy-Weinberg equlibrium (P>0.05) at the two sites. The cashmere production, cashmere production per body surface area, birth weight, gaining weight from birth to weanling among different genotypes had no significant difference in White Cashmere goats (P>0.1), although allele A had a trend to increase cashmere production Goat Dkk1 is homologous to other mammal species. Most of the polymorphisms were in noncoding region of goat Dkk1 gene. No significant association was found between the T593C and C603A polymorphisms in Dkk1 promoter region and cashmere production