鹅IDH1基因的分离、序列分析及表达特征研究

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (7): 992-998.doi:

鹅IDH1基因的分离、序列分析及表达特征研究

赵阿勇^1，2，陈国宏^1* ，卢立志^3* ，周圻²

1.扬州大学动物科学与技术学院，扬州 225009；2浙江林学院动物科学系，临安 311300；3浙江省农业科学院畜牧兽医研究所，杭州 310021

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-07-24 发布日期:2009-07-24

Isolation, Sequence Analysis and Expression Profile of IDH1 in Goose Liver

ZHAO Ayong ^{1, 2} , CHEN Guohong ^1* , LU Lizhi ^3* , ZHOU Qi²

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 2. Department of Animal Science, Zhejiang Forestry University, Linan 311300, China;3. Institute of Animal Science and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:1900-01-01 Revised:1900-01-01 Online:2009-07-24 Published:2009-07-24

摘要/Abstract

摘要： 为阐明鹅肥肝形成的机制，使用mRNA差异显示技术研究朗德鹅和溆浦鹅在超饲养和正常饲养条件下肝脏基因表达差异。基因IDH1被证实在2个品种鹅肝脏中表达受到显著抑制（P<005）。结果，得到了该基因1 269 bp的CDS序列，与鸡IDH1有95%的同源性。序列分析表明，该序列含有1个1 248 bp的开放读码框（ORF），编码含415个氨基酸的蛋白质，该蛋白质序列存在1个保守结构域Icd，与其它物种该蛋白的同源性分别为：鸡99%、大鼠89%、人90%、猿90%、牛88%、小鼠88%。应用生物信息学的方法对该蛋白质的功能和结构进行了分析。组织表达分析表明，鹅IDH1基因在肝脏中表达丰富，在脾、肾和肌胃中中等表达，在其它组织中表达量较低。研究结果显示，超饲可以抑制鹅肝脏IDH1基因mRNA的表达。

Abstract: To obtain an understanding of fattening mechanism of fatty liver, mRNA differential display reverse transcription PCR (DDRTPCR) was applied to study the differences of gene expression in French Landes Grey goose and Xupu White goose in different conditions of overfeeding and normal feeding. One gene was found to be significantly downregulated in fatty liver in both breeds（P<005）, which has a CDS length of 1 269 bp and 95% identity to chicken IDH1. The sequence analysis revealed that its 1 248 bpinlength open reading frame (ORF) encodes a 415aminoacids protein containning a putative conserved domain of Icd and has high homology with chicken(99%), rat(89%), human(90%), monkey(90%), cattle(88%),and mouse(88%)，respectively. The structure and function of this protein were analyzed using bioinformatics methods.The tissue expression analysis indicated that goose IDH1 mRNA was higher expressed in liver than that in other tested tissues. The present results suggested that overfeeding could decrease the mRNA expression level of goose IDH1.

赵阿勇;陈国宏;卢立志;周圻. 鹅IDH1基因的分离、序列分析及表达特征研究[J]. 畜牧兽医学报, 2009, 40(7): 992-998.

ZHAO Ayong;;CHEN Guohong ;LU Lizhi ;ZHOU Qi . Isolation, Sequence Analysis and Expression Profile of IDH1 in Goose Liver[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(7): 992-998.

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