肉牛PRKAG3基因多态性及其与胴体和肉质性状的关联分析

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (7): 1106-1111.doi:

肉牛PRKAG3基因多态性及其与胴体和肉质性状的关联分析

李武峰¹，杨润军³，甘乾福²，张路培²，许尚忠²*，李俊雅²，高雪²

1.山西农业大学动物科技学院，太谷 030801；2.中国农业科学院北京畜牧兽医研究所，北京 100193；3.西北农林科技大学动物科技学院，杨凌 712100

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-07-24 发布日期:2009-07-24
通讯作者: 许尚忠

Polymorphism of PRKAG3 Gene and Its Association with Carcass and Meat Quality Traits in Beef Cattle

LI Wufeng ¹, YANG Runjun ³, GAN Qianfu², ZHANG Lupei ²,
XU Shangzhong²*, LI Junya ², GAO Xue²

1.College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China; 2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China；3. College of Animal Science and Technology, Northwest Agricultural and Forestry University, Yangling 712100,China

Received:1900-01-01 Revised:1900-01-01 Online:2009-07-24 Published:2009-07-24
Contact: XU Shangzhong

摘要/Abstract

摘要： 本研究以晋南牛、鲁西牛、西门塔尔、安格斯、海福特、利木赞和夏洛莱7个品种共267头肉牛为试验材料，采用PCRRFLP技术检测PRKAG3基因多态性，找到了PRKAG3基因DNA序列中的 2个突变位点T2643C和T2885C(DQ082736)，同属第4内含子。2个基因多态性位点与肉牛胴体和肉质共9个性状的关联分析表明：PRKAG3基因T2885C变异位点的3个基因型间嫩度性状差异显著（P＜005），CC基因型最小二乘均值显著低于CD和DD基因型最小二乘均值。本研究结果可为肉牛肉质性状标记辅助选择提供依据。

Abstract: Polymorphisms of PRKAG3 gene were detected in 267 beef cattle in 7 beef cattle breeds (Simmental, Angus, Hereford, Charolais, Luxi cattle, Limousin and Jinnan cattle) by PCRRFLP. Two polymorphic sites T2643C and T2885C were found(DQ082736), both of them in intron 4. Association analysis of the two SNPs with 9 carcass and meat quality traits was carried out. The results showed that T2885C substitution of PRKAG3 gene is significantly associated(P＜005) with meat tenderness, and the mean value of individuals with genotype CC is lower than that of individuals with genotype CD and DD. The information found in the present research is very important for meat quality breeding in beef cattle by markerassisted selection.

李武峰;杨润军;甘乾福;张路培;许尚忠;李俊雅;高雪. 肉牛PRKAG3基因多态性及其与胴体和肉质性状的关联分析[J]. 畜牧兽医学报, 2009, 40(7): 1106-1111.

LI Wufeng;YANG Runjun;GAN Qianfu;ZHANG Lupei;XU Shangzhong;LI Junya;GAO Xue . Polymorphism of PRKAG3 Gene and Its Association with Carcass and Meat Quality Traits in Beef Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(7): 1106-1111.

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