Abstract: VP1 gene was successfully cloned by RTPCR from DHV1 of strain 161/79/V, YH and HN. Phylogenetic and evolutionary analysis of VP1 gene revealed that the homology of the nucleic acid and amino acid sequences were 927%-969% and 954%-966% between the three strains and DHV1 respectively, and were below 75% and 88% between the three strains and the mutant strains. The three strains were belong to DHV1 and were different from mutant strains. The amino acid sequences of virulent strain 161/79/V of DHV1 were T49 and H 183, which mutated as S49 and Q183 in avirulent strain YH and strain HN. The mutation would potentially lead to the changes of virulence. DHV1 and mutant strains lacked the conservative peptide motif RGD. Strain 90 and strain 04G of NDHV procured two more amino acids Q50 and D51 than DHV1,but lost two amino acids, E147 and L185.The strains AP03337, AP04009， AP04114 and AP04203 of DHV procured two more amino acids G145 and G146 than DHV1. Different serotypes of DHV had different antigenic index at 4664aa, 95149aa and 180223aa, which impacted on biological characteristic potentially.