Abstract: Fortyweek healthy Sanhuang (SH) broilers were randomly divided into floorreared （treatment） and cagereared groups (control), and feed with the same diet. During the experimental period, 300 fertile eggs were randomly collected from each group and directly incubated in an electric forceddraft incubator. Livers were collected on day9, 14, 19 of incubation and the hatching, respectively. Proteins were extracted and separated by 2DPAGE and stained with colloidal Coomassie Brilliant Blue. Then, the stained gels were scanned and analyzed using PDQuest73 software. The differential protein spots with ingel trypsin digestion were subsequently identified by MALDITOFMS. The results indicated that the embryo and absolute liver weight of floorreared group was significantly lower than that of the control at hatching, although no significant differences were observed in both embryo and absolute liver weight between the floorreared group and the control on day 9, 14 and 19 of embryonic development. The result of offspring broiler liver protein profiles showed that twentynine proteins had expressed at least 2.0fold variance compared to the control during embryonic development and twentytwo of them were identified. Most of these proteins were highly related to carbohydrate metabolism, energy production and signal transduction.