Abstract: Total RNA extracted from the uterus of Jining gray goat was used for RT-PCR amplification of goat luteinizing hormone receptor (LHR) gene. A cDNA fragment of 289 bp in size was obtained and cloned into pGEM-T vector. Positive clones were identified and sequenced. Using GAPDH as an inner control, an optimal semi-quantitative RT-PCR method was established to analyze LHR mRNA expression levels in the goat uterus of four estrous cycle phases. Sequencing analysis indicated that this fragment was a partial sequence of LHR cDNA and identical to the reported LHR mRNA (AF379199). The LHR mRNA expression was different in the uterus of Jining gray goat during the four estrous cycle phases, being lowest in the estrus (0.454±0.06), highest in the diestrus (0.705±0.09), intermediate in the proestrus (0.553±0.07).The expression in the estrus was significantly lower than that in other oestrous cycle phases (P<0.05).The results provides molecular data for further elucidating the mechanisms of reproductive endocrine secretion in goats.