日粮高硒对雏鸡外周血T淋巴细胞增殖功能和亚群的影响

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (2): 256-260.doi:

日粮高硒对雏鸡外周血T淋巴细胞增殖功能和亚群的影响

彭西，崔赟，杨帆，崔伟，崔恒敏*

四川农业大学动物医学院，雅安 625014

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-02-24 发布日期:2009-02-24
通讯作者: 崔恒敏

Effects of High Dietary Selenium on the Proliferation Function and Subset of Peripheral Blood T Lymphocyte in Chickens

PENG Xi, CUI Yun,YANG Fan, CUI Wei, CUI Heng-min*

College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-24 Published:2009-02-24

摘要/Abstract

摘要： 300只1日龄艾维茵肉鸡健雏随机分为5组，分别喂以基础日粮(日粮Se含量0.2 mg·kg^-1，对照组)和高硒日粮（Se 1 mg·kg^-1，高硒Ⅰ组；Se 5 mg·kg^-1，高硒Ⅱ组；Se 10 mg·kg^-1，高硒Ⅲ组；Se 15 mg·kg^-1，高硒Ⅳ组）6周，用流式细胞术和MTT法研究高硒对外周血T淋巴细胞亚群、淋巴细胞增殖功能的影响。结果显示，当雏鸡日粮硒水平达5 mg·kg^-1以上时，外周血CD3⁺T细胞、CD3⁺CD4⁺T细胞和CD3⁺CD8⁺T细胞百分比不同程度地降低；高硒Ⅲ、Ⅳ组的T淋巴细胞增殖能力下降，血清IL-2含量降低，与对照组间差异显著或极显著（P<0.05或P<0.01）。结果表明，5 mg·kg^-1以上的日粮硒降低雏鸡细胞免疫功能。

Abstract: By the methods of FCM and MTT, the subsets and lymphocyte proliferation function of peripheral blood T lymphocyte were studied in AA poultries by feeding with sufficient (0.2 mg·kg^-1, control group), or excess selenium (1, 5, 10, and 15 mg·kg^-1), as sodium selenite in the diets to five groups of 60 chickens each for 6 weeks. The results showed that the percentage of CD3⁺ T cells, CD3⁺CD4⁺ T cells and CD3⁺CD8⁺ T cells were decreased variably in chickens when dietary selenium exceeded 5 mg·kg^-1. The mitogenic response of T lymphocytes to ConA and the content of serum IL-2 were decreased in high Se group Ⅲ and high Se group Ⅳ (P<0.05 or P<0.01) when compared with control group. The results suggested that the dietary selenium in excess of 5 mg·kg^-1 impaired the cellular immune function in chickens.

彭西;崔赟;杨帆;崔伟;崔恒敏. 日粮高硒对雏鸡外周血T淋巴细胞增殖功能和亚群的影响[J]. 畜牧兽医学报, 2009, 40(2): 256-260.

PENG Xi;CUI Yun;YANG Fan;CUI Wei;CUI Heng-min. Effects of High Dietary Selenium on the Proliferation Function and Subset of Peripheral Blood T Lymphocyte in Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(2): 256-260.

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