朗德鹅FAS基因RFLP与屠宰性状、产肝性状及脂肪沉积性状的相关分析

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (2): 166-172.doi:

朗德鹅FAS基因RFLP与屠宰性状、产肝性状及脂肪沉积性状的相关分析

邝智祥¹，何大乾²*，刘益平¹，朱庆¹*

1.四川农业大学动物科技学院，雅安 625014; 2.上海市农业科学院畜牧兽医研究所，上海 201106

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-02-24 发布日期:2009-02-24
通讯作者: 朱庆;何大乾

Study on Association of Restriction Fragment Length Polymorphisms of Fatty Acid Synthase Gene with Carcass， Liver Performance and Fat Deposit Traits in Landes Goose

KUANG Zhi-xiang¹，HE Da-qian²*，LIU Yi-ping¹，ZHU Qing¹*

1.College of Animal Science and Technology， Sichuan Agricultural University， Ya′an 625014， China;2.Institute of Animal Husbandry and Veterinary Medicine， Shanghai Academy of Agricultural Sciences， Shanghai 201106， China

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-24 Published:2009-02-24

摘要/Abstract

摘要： 采用PCR-RFLP技术，分析脂肪酸合成酶（FAS）基因启动子区在朗德鹅中的AfaⅠ酶切片段多态性分布。FAS基因启动子区具有3个AfaⅠ酶切位点（位点A、B和E），3个位点在朗德鹅中均具有多态性。通过χ2检验显示3个酶切位点都不符合哈代温伯格法则。最小二乘分析结果显示：E位点中FASEE基因型个体在活体质量、填饲期增质量、屠体质量、半净膛质量、全净膛质量、胸肌质量、腿肌质量方面比FASee基因型个体高出12.78%～19.67%（P＜0.05）；位点B具有类似的影响趋势；而位点A对屠宰性能没有显著影响（除了胸肌质量）（P >0.05）。在3个酶切位点中，位点A和B的杂合基因型FASAa和FASBb个体产肝性能比纯合基因型好，而E位点的FASEE基因型个体肝质量比FASee基因型个体高33.09％（P＜0.05）。3个酶切位点对体脂沉积性状影响均不显著。

Abstract: The aim of this study was to investigate polymorphism of fatty acid synthase (FAS) gene promoter region in Landes goose. Polymorphisms were detected by restriction fragment length polymorphisms(RFLP) and enzyme AfaⅠwas used. Three AfaⅠdigestion sites (locus A， B and E) were detected in Landes goose. The χ²-test was performed to examine the HardyWeinberg equilibrium (HWE). Three sites significantly deviated from HWE. The least square analysis showed that living weight， weight gain at feeding increase stage， carcass weight， halfeviscerate weight， eviscerate weight， breast muscle weight and leg muscle weight of individuals with genotype FAS^EE were higher than that of individuals with genotype FAS^ee 12.78%-19.67% （P＜0.05） at loci E and B. Locus A had no significant effects on carcass traits except for breast muscle weight（P＞0.05）. Fatty liver performance of individuals with FASAa and FAS^Bb were better than that of individuals with homozygote at loci A and B， but the liver weight of individuals with genotype FAS^EEwas higher than that of individuals with heterogenesis 33.09％（P＜0.05）at locus E. Three loci had no significant effects on fat deposit traits.

邝智祥;何大乾;刘益平;朱庆 . 朗德鹅FAS基因RFLP与屠宰性状、产肝性状及脂肪沉积性状的相关分析[J]. 畜牧兽医学报, 2009, 40(2): 166-172.

KUANG Zhi-xiang;HE Da-qian;LIU Yi-ping;ZHU Qing. Study on Association of Restriction Fragment Length Polymorphisms of Fatty Acid Synthase Gene with Carcass， Liver Performance and Fat Deposit Traits in Landes Goose[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(2): 166-172.

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