鸡MyoT基因组织表达谱分析、SNP检测及其与胴体和肉质性状的关联分析

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (2): 161-165.doi:

鸡MyoT基因组织表达谱分析、SNP检测及其与胴体和肉质性状的关联分析

朱志明^1,2，陈国宏³，强巴央宗⁴，李长春¹，朱猛进¹，樊斌¹，刘榜¹*

1. 华中农业大学动物科技学院动物分子生物学与育种实验室,武汉430070；2. 福建省农科院畜牧兽医研究所，福州350013； 3. 扬州大学动物科技学院，扬州225009; 4. 西藏大学农牧学院，林芝860000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-02-24 发布日期:2009-02-24
通讯作者: 刘榜

Tissue Distribution, SNP Detection and Its Association Analysis with Carcass and Meat Quality Traits of Chicken MyoT Gene

ZHU Zhi-ming^1,2, CHEN Guo-hong³, QIANGBA Yang-zong⁴, LI Chang-chun¹,
ZHU Meng-jin¹, FAN Bin¹, LIU Bang¹*

1.Lab of Molecular Biology and Animal Breeding,College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070，China；2. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences,Fuzhou 350013，China; 3. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009，China;4. College of Agriculture and Animal Husbandry, Tibet University, Linzhi 860000，China

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-24 Published:2009-02-24
Contact: LIU Bang

摘要/Abstract

摘要： 利用半定量RT-PCR方法，对MyoT基因在白来航鸡7种不同组织（心、肝、脾、肺、肾、脑和肌肉）中的表达谱进行研究。结果表明，MyoT基因只在心脏和肌肉中表达。克隆得到了鸡MyoT基因的部分片段，发现外显子10存在A/G转换，利用PCR-Hin6Ⅰ-RFLP方法对其在12个中国地方鸡种（白耳鸡、仙居鸡、大骨鸡、鹿苑鸡、固始鸡、斗鸡、乌骨鸡、油鸡、茶花鸡、萧山鸡、狼山和藏鸡）和1个外来鸡种（隐性白羽鸡）中的分布进行了研究。在藏鸡、隐性白羽鸡、杂交鸡(藏♂×隐♀)和75%血液藏鸡\[藏♂×(藏♂×隐♀)♀\]群体中进行了不同基因型与性状间的关联分析，结果表明，该SNP位点不同基因型与腿肌质量显著相关（P<0.05），与腿肌率和腿肌肉色极显著相关（P<0.01）。

Abstract: The semi-quantitative RT-PCR was performed to detect the expression pattern of chicken MyoT gene in 7 different tissues (heart, liver, spleen, lung, kidney, brain and muscle). The results showed that MyoT gene was expressed only in heart and muscle. Partial DNA sequence of chicken MyoT gene was cloned and a A/G transition was found in exon 10. Using PCR-Hin6Ⅰ-RFLP method, the A/G SNP locus was analyzed in 12 native chicken breeds (White Ear, Xianju, Dagu, Luyuan, Gushi, Game chickens, Silky Sooty, Beijing Fatty, Chahua, Xiaoshan, Langshan and Tibetan chicken) and 1 foreign breeds (Recessive White chicken). The association between different genotypes and carcass and meat quality traits was analyzed in Tibetan chicken, Recessive white, hybrid chicken (Tibetan♂×Recessive♀). The result of association analysis showed there were correlation between this SNP site and leg muscle weight (P＜0.05), and significant correlation between this SNP site and leg muscle rate and meat colour (leg muscle) (P＜0.01).

朱志明;陈国宏;强巴央宗;李长春;朱猛进;樊斌;刘榜. 鸡MyoT基因组织表达谱分析、SNP检测及其与胴体和肉质性状的关联分析[J]. 畜牧兽医学报, 2009, 40(2): 161-165.

ZHU Zhi-ming;CHEN Guo-hong;QIANGBA Yang-zong;LI Chang-chun;ZHU Meng-jin;FAN Bin;LIU Bang. Tissue Distribution, SNP Detection and Its Association Analysis with Carcass and Meat Quality Traits of Chicken MyoT Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(2): 161-165.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/

https://www.xmsyxb.com/CN/Y2009/V40/I2/161