畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (8): 1137-1141.doi:

• 研究简报 • 上一篇    下一篇

山羊线粒体DNA D-loop区部分序列变异位点分析

武艳平1,2,关伟军1,赵倩君1,何晓红1,浦亚斌1,霍俊宏2,敖红1,李奎1,马月辉1   

  1. 1.中国农业科学院北京畜牧兽医研究所,北京 100193;2.江西农业科学院畜牧兽医研究所,南昌 330200
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-08-24 发布日期:2008-08-24
  • 通讯作者: 马月辉

Analysis on Partial Sequence of Mitochondrial DNA D-loop Region Variation Position of Goat

WU Yan-ping1,2,GUAN Wei-jun1,ZHAO Qian-jun1,HE Xiao-hong1,PU Ya-bin1,HUO Jun-hong2,AO Hong1,LI Kui1,MA Yue-hui1   

  1. 1.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193,China;2.Institute of Animal Science,Jiangxi Academy of Agricultural Sciences, Nanchang 330200,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-08-24 Published:2008-08-24
  • Contact: MA Yue-hui

摘要: 截取了山羊线粒体DNA D-loop高变区453 bp的部分片段(线粒体全序列的15 735~16 187 bp处),共计1 182条序列进行了多重比对,并进行系统建树和网络关系分析,结果这些序列明显的分成了4个支系(A、 B、 C、 D),分别含有1 001、134、24和13条序列,进一步比对分析,发现这4个支系有各自特有的碱基变异特点,A、B、C和D分别有5、6、18和7个特有的碱基变异位点,除了各个支系特有的变异外,还发现有2个支系共有的变异位点。

Abstract: In this study, we aligned and analyzed 453 bp segment of mitochondrial DNA D-loop hypervariable region (Positions 15 735-16 187 bp of complete mitochondrial genome) from 1 182 sequences, neighbor-joining (NJ) tree and network showed that the sequences were divided into four distinct mtDNA lineages A-D,and included 1 001,134,24 and 13 sequences, respectively.After the alignment of the sequences,nucleotide position of A,B, C and D lineage specific were acquired, and included 5,6,18 and 7 variation positions, respectively, moreover, 2 lineages common variation positions were found.