猪冷冻精液体外受精研究

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (8): 1045-1049.doi:

猪冷冻精液体外受精研究

丁义^1，2，3 ，戴建军^1,2 ，吴华莉^1,2 ，张德福^1,2 *，徐刚毅³

1. 上海农业科学院兽医研究所，上海 201106；2.上海农业遗传育种重点实验室动物遗传工程研究室，上海 201106；3. 四川农业大学动物科技学院，雅安 625014

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-08-24 发布日期:2008-08-24
通讯作者: 张德福

Studies on in vitro Fertilization of Porcine Frozen Semen

DING Yi^1,2,3, DAI Jian-jun^1,2,WU Hua-li^1,2，ZHANG De-fu^1,2*, XU Gang-yi³

1.Institute of Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106,China; 2.Shanghai Municipal Key Laboratory of Agrigenetics and Breeding, Shanghai 201106, China;3.College of Animal Science, Sichuan Agricultural University,Ya’an 625014, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-08-24 Published:2008-08-24
Contact: ZHANG De-fu

摘要/Abstract

摘要： 为简化猪体外受精过程中精液的来源，避免不同公猪精液和不同采精时间对猪体外受精的影响，本研究采用5 mL大管冷冻精液进行体外受精（IVF），研究IVF过程中的各项参数。结果表明：精卵共孵2~8 h对多精入卵率影响不大 (P>0.05)， 2-细胞率随着共孵时间的延长而增加，8 h时达到72.8%，显著高于2 h组(60.5%，P<0.05)，桑椹胚和囊胚发育率以6 h组最高，分别达46.1%和3.48%。多精入卵率随精子浓度的增加而增加，且各组之间差异显著(P<0.05)，2-细胞率也以108组(81.2%)最高，且显著高于105组(63.7%)(P<0.05)，同时107组和106组分别获得了8.11%和3.13%的囊胚发育率。以NCSU-23作为成熟培养液，并部分去除卵丘细胞的IVF卵裂率和囊胚率最高，达77.1%和7.33%。冻精和新鲜精液的IVF，无论是在卵裂率，还是桑椹胚或囊胚率上均无显著差异(P>0.05)。

Abstract: In order to simplify the source of semen of in vitro fertilization(IVF), and avoid the effects by different boars and different semen collection time, we used frozen semen by 5 mL-straw to do the IVF. The results showed that there were no significant differences in the polyspermy rates among the 2-8 h oocyte-sperm incubation time(P>0.05). The 2-cell rate was improved along with the increasing of oocyte-sperm incubation time, and 8 h group (72.8%) was much higher than the 2 h group(60.5%, P<0.05). The morula and blastocysts rates of the 6 h group were the highest and got 46.1% and 3.48%, respectively. Polyspermy rate was also increased with the increasing of sperm concentration in the IVF (P<0.05). The 2-cell rate of 108 group(81.2%) was the highest, which was much higher than the 105 group. The 107 and 106 group got 8.11% and 3.13% blastocyst rate, respectively. Using NCSU-23 as the in vitro maturation(IVM) medium and matured oocytes partly denuded cumulus cells could get the highest cleavage and blastocyst rates(77.1%,7.33%) among the four groups. The comparation between the frozen semen and fresh semen in the IVF showed that there were no significant differences in the cleavage, morula and blastocyst rates(P>0.05).

丁义;;戴建军;;吴华莉;;张德福;;徐刚毅 . 猪冷冻精液体外受精研究[J]. 畜牧兽医学报, 2008, 39(8): 1045-1049.

DING Yi;;DAI Jian-jun;WU Hua-li;ZHANG De-fu;XU Gang-yi. Studies on in vitro Fertilization of Porcine Frozen Semen[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(8): 1045-1049.

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