FSH通过激活ERK1/2调节仔猪睾丸支持细胞GDNF的表达

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (8): 1040-1044.doi:

FSH通过激活ERK1/2调节仔猪睾丸支持细胞GDNF的表达

孙燕^1，2 ，王鲜忠^1，2，吴建云¹ ,白汝岚¹,张家骅^1，2

1.西南大学牧草与草食家畜重点实验室,重庆 400716；2.西南大学动物科技学院,重庆 400716

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-08-24 发布日期:2008-08-24
通讯作者: 张家骅

FSH Regulating the Expression of GDNF of Piglet Testis Sertoli Cellby Activing ERK1/2

SUN Yan^1,2,WANG Xian-zhong^1,2, WU Jian-yun¹, BAI Ru-lan¹, ZHANG Jia-hua^1,2

1.Chongqing Key Laboratory of Forage & Herbivore, Southwest University,Chongqing 400716,China；2.College of Animal Science and Technology,Southwest University,Chongqing 400716，China

Received:1900-01-01 Revised:1900-01-01 Online:2008-08-24 Published:2008-08-24
Contact: ZHANG Jia-hua

摘要/Abstract

摘要： 以体外培养的仔猪睾丸支持细胞为研究对象，研究了FSH对GDNF蛋白的调节及其信号传导机制。结果显示：（1）外源性FSH以浓度和时间依赖性促进GDNF蛋白表达：FSH浓度为50 ng/mL、作用1 h 时，GDNF水平达到最高；（2）用FSH（50 ng/mL）或dbcAMP（100 μmol/L）处理支持细胞，可迅速激活MEK激酶，随FSH刺激时间的延长，ERK1/2活性逐渐升高（0~2 h）；（3）MEK1/2的抑制剂U0126可显著抑制FSH对GDNF的激活作用。结果表明，FSH在诱导GDNF蛋白表达的过程中，通过cAMPPKAERK1/2级联通路，促进GDNF基因的表达。

Abstract: Primary piglet sertoli cell (SC) were used to study the effects of FSH on regulating Glial cell linederived neurotrophic factor (GDNF) expression. The results showed that: (1) The FSH-stimulated GDNF expression in the cultured SC was time- and concentration-dependent: the level of GDNF protein reached its highest when FSH (50 ng/mL) stimulated 1h. (2) FSH（50 ng/mL）or dbcAMP（100 μmol/L）could active ERK1/2 prominently along with a maximum stimulation at 2 h; (3) U0126 (MEK1/2 inhibitor) could apparently inhibited the activing effect of FSH on GDNF. These results suggested that, by activing the cAMPPKAERK1/2 and promting the transcription of GDNF gene, FSH stimulated the expression of GDNF of sertoli cells.

孙燕;;王鲜忠;吴建云;白汝岚;张家骅;. FSH通过激活ERK1/2调节仔猪睾丸支持细胞GDNF的表达[J]. 畜牧兽医学报, 2008, 39(8): 1040-1044.

SUN Yan;WANG Xian-zhong;WU Jian-yun;BAI Ru-lan;ZHANG Jia-hua;. FSH Regulating the Expression of GDNF of Piglet Testis Sertoli Cellby Activing ERK1/2[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(8): 1040-1044.

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